Nutritional composition and proteomic analysis of soft-shelled turtle (Pelodiscus sinensis) egg and identification of oligopeptides with alpha-glucosidase inhibitory activity.

Food Research International
Leyun QiuLiufeng Zheng

Abstract

This study aimed to explore nutritional compositions and proteomics of soft-shelled turtle (SST) egg, as well as identify potential antidiabetic oligopeptides with α-glucosidase inhibitory property. Results revealed that SST egg is a promising source of highly nutritious proteins and minerals (54.64% and 5.81% of dry matter, respectively). Further proteomic analysis showed SST egg proteins contained at least 9 protein families, such as transferrin/iron binding protein and immunoregulation-related protein. Hydrolysis by different enzymes, especially papain, remarkably increased α-glucosidase inhibitory activity and scavenging activity for ABTS, DPPH, hydroxyl and oxygen radicals of SST egg proteins. Peptides from papain hydrolysate were fractionated using ultrafiltration followed by reverse phase chromatography, and 16 peptides were identified in the most active fraction by LC-QTOF-MS/MS. Molecular docking revealed that 14 of these peptides could easily dock into the substrate-binding pocket and/or inhibitor binding sites of α-glucosidase with the docking score below -150 kcal/mol, indicating their potential α-glucosidase inhibitory properties. The five most abundant oligopeptides with potent interaction with α-glucosidase were ...Continue Reading

References

Oct 27, 2005·Journal of Agricultural and Food Chemistry·Jennifer Kovacs-NolanYoshinori Mine
Jun 5, 2010·Advances in Physiology Education·Barbara E Goodman
Nov 19, 2011·Pharmacognosy Reviews·Sunil KumarOm Prakash
Jan 24, 2012·Journal of Proteomics·Bung-on PrajanbanSompong Thammasirirak
Jan 1, 1972·Methods in Enzymology·R Fields
Feb 6, 2013·Diabetes·Jennifer E HoThomas J Wang
Dec 18, 2013·International Journal of Biological Macromolecules·Jiakai YanYajie Wang
May 22, 2016·Diabetes Care·Marta Guasch-FerréFrank B Hu
Nov 30, 2016·International Journal of Biological Macromolecules·Lin HanMin Wang
Dec 13, 2016·The Biochemical Journal·Philip NewsholmePaulo Ivo Homem de Bittencourt
Feb 1, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Gabrielli Barbosa de CarvalhoLiliane Viana Pires
Feb 2, 2017·American Journal of Physiology. Endocrinology and Metabolism·Colleen S DeanePhilip J Atherton
Feb 6, 2017·Biochimica Et Biophysica Acta. Biomembranes·Bung-On PrajanbanSompong Klaynongsruang
Jun 14, 2017·Critical Reviews in Food Science and Nutrition·Ya-Fei LiuPat Silcock
Sep 9, 2017·Critical Reviews in Food Science and Nutrition·José Manuel Fernández RealMelania Manco
Oct 13, 2017·European Journal of Nutrition·Jayapal Naveen, Vallikannan Baskaran
May 11, 2018·Nucleic Acids Research·Pei ZhouSheng-You Huang
Jun 15, 2018·Journal of Agricultural and Food Chemistry·Huafang DingGuowen Zhang
Aug 22, 2018·Journal of Intensive Care Medicine·George Sam Wang, Christopher Hoyte
Apr 9, 2019·Food Chemistry·Magdalena MontowskaEmilia Fornal
Apr 10, 2019·Current Vascular Pharmacology·Dragan LovicAthanasios Manolis
Jun 20, 2019·Frontiers in Endocrinology·Manjunath RamanjaneyaAbdul-Badi Abou-Samra
Dec 29, 2019·The Journal of Nutrition·Robert L BeverlyDavid C Dallas
Mar 28, 2020·Oxidative Medicine and Cellular Longevity·Habib YaribeygiAmirhossein Sahebkar
Apr 24, 2020·Journal of Chemical Theory and Computation·Gaoqi WengTingjun Hou
May 14, 2020·Nature Reviews. Nephrology·Joanne B Cole, Jose C Florez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.