Nutritional regulation of the glucose-6-phosphate dehydrogenase gene is mediated by a nuclear posttranscriptional mechanism

Archives of Biochemistry and Biophysics
D L Hodge, Lisa M Salati

Abstract

Expression of the glucose-6-phosphate dehydrogenase (G6PD) gene is inhibited by addition of polyunsaturated fat to a high-carbohydrate diet and stimulated by feeding a high-carbohydrate diet to starved mice. The mechanism of this regulation is posttranscriptional. To define the regulated step, we measured the abundance of G6PD mRNA both in the nucleus and in total RNA. Feeding mice a high-fat diet results in a 70% or greater inhibition of nuclear precursor mRNA (pre-mRNA) and mature mRNA abundance. Amounts of both pre-mRNA and mature mRNA for G6PD are stimulated 13-fold or more by refeeding starved mice. Changes in amount of pre-mRNA for G6PD are of a similar magnitude and precede the changes in amount of mature mRNA for G6PD in total RNA. These changes in pre-mRNA abundance occur in the absence of observable changes in the rate of transport of mRNA from the nucleus to the cytoplasm, splicing of the pre-mRNA, or degradation at the 3'-end of the transcript. Despite large changes in pre-mRNA amount in mice fed a low-fat diet relative to mice fed a high-fat diet, the rate of change in the amount of pre-mRNA during the diurnal feeding cycle is not altered. Thus, expression of G6PD is regulated at an early step after transcription o...Continue Reading

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Citations

Aug 17, 1999·Annual Review of Nutrition·D B Jump, S D Clarke
May 26, 2001·Annual Review of Nutrition·L M Salati, B Amir-Ahmady
Dec 28, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wilma M FrederiksCornelis J F Van Noorden
Jun 20, 2002·The Journal of Biological Chemistry·Huimin TaoLisa M Salati
Oct 8, 2005·The Journal of Biological Chemistry·Indrani TalukdarLisa M Salati
Sep 19, 2006·The Journal of Biological Chemistry·Wioletta Szeszel-FedorowiczLisa M Salati
Jan 2, 2001·The Journal of Biological Chemistry·B Amir-Ahmady, L M Salati
Aug 31, 2004·The Journal of Nutrition·Lisa M SalatiDeborah L Hodge
May 20, 2021·Oxidative Medicine and Cellular Longevity·Maria Pina DoreGiovanni Mario Pes

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