Apr 1, 1976

Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores

Journal of Bacteriology
C F Hirsch, J C Ensign

Abstract

Spores of Streptomyces viridochromogenes were removed from the surface of solid media with glass beads and suspended in a buffer-detergent solution. Addition of yeast extract and glucose resulted in rapid loss of refractility of the spores. Appearance of germ tubes followed. Germination was accompanied by a decrease in the optical density (OD) of the suspension. The OD decrease was used as an assay for germination. A defined germination medium (DGM) comprised of L-alanine, L-glutamic acid, adenosine, para-aminobenzoic acid, and calcium and magnesium ions provided a germination rate nearly equal to that of complex media. The germination rate was essentially the same if D-alanine and D-glutamate replaced the L-isomers. The optimum pH and temperature for germination were 7.0 and 35 C. Germination was absolutely dependent on the presence of CO2. Spores harvested after growth for longer periods than the usual time (10 days) became less germinable in DGM. The same was observed for spores grown at 37 C as compared with 30 C. Spores incubated in DGM for various time periods before being transferred to a buffer solution did not continue to germinate. Spores harvested after growth of eight species of Streptomyces did not show a decrease ...Continue Reading

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Mentioned in this Paper

Molecular Stereochemistry
Adenosine
Calcium
Spores, Bacterial
Reproduction Spores
Glucose, (beta-D)-Isomer
Abufne
Germination
D-Glutamate
Aminobenzoic Acids

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