Nylon filament coated with paraffin for intraluminal permanent middle cerebral artery occlusion in rats

Neuroscience Letters
Xia-Lin ZuoAi-Min Ji

Abstract

A variety of intraluminal nylon filament has been used in rat middle cerebral artery occlusion (MCAO) models. However the lesion extent and its reproducibility vary among laboratories. The properties of nylon filament play a part of reasons for these variations. In the present study, we used paraffin-coated nylon filament for rat MCAO model, tested the effects and advanced improvement for making the rat MCAO. Forty male Sprague-Dawley (SD) rats were randomized into two groups, MCAO with traditional uncoated nylon filament (uMCAO) and MCAO with paraffin-coated nylon filament (cMCAO), three rats as normal group and sham group respectively. Assessment included mortality rates, model success rates, neurological deficit evaluation, and infarct volume. The study showed two rats died in uMCAO group, no rat died in cMCAO group within the 12h. The model success rate of uMCAO was 100%, while the uMCAO group was 55% (n=20, two died within 12h, seven rats were excluded as the brain slices showed no TTC staining due to subarachanoid hemorrhage). Neurological evaluation demonstrated group cMCAO had more worse neurological outcomes than group uMCAO, and the difference was statistically signification (p<0.05). TTC staining cMCAO group had sign...Continue Reading

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Citations

Jan 13, 2015·Molecular Medicine Reports·Houliang DengAimin Ji
Sep 25, 2015·Neuroscience Letters·Wen-hao Sun, Hui-Sheng Chen
Jun 26, 2013·Neuropathology and Applied Neurobiology·Levente KnappTamás Farkas
Feb 4, 2016·Scientific Reports·Edvin IngbergJakob O Ström
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kai LiuLei Liu
Aug 14, 2015·The International Journal of Neuroscience·Xia-Lin ZuoEn Xu

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