O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates

Environmental Microbiology
Hans WildschutteMartin F Polz

Abstract

The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyp...Continue Reading

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Citations

Aug 30, 2011·Applied and Environmental Microbiology·Sarah P PreheimMartin F Polz
Jan 22, 2013·Trends in Genetics : TIG·Martin F PolzWilliam P Hanage
Apr 4, 2016·Trends in Microbiology·Frédérique Le RouxMartin F Polz
Apr 15, 2016·FEMS Microbiology Reviews·Rita F MaldonadoMiguel A Valvano
Mar 5, 2014·Nature Reviews. Microbiology·Otto X Cordero, Martin F Polz
Nov 25, 2016·Applied and Environmental Microbiology·Payel ChatterjeeHans Wildschutte
May 5, 2020·Environmental Microbiology·Delphine Destoumieux-GarzónLuigi Vezzulli
Jun 14, 2018·Frontiers in Microbiology·Andrew WagnerHans Wildschutte
Mar 29, 2020·Environmental Microbiology·Daniel OyanedelDelphine Destoumieux-Garzón

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