O-GlcNAc mediated glycosylation down-regulation in mice with cyclophosphamide induced cystitis.

The Journal of Urology
Seyung ChungChester J Koh

Abstract

Cyclophosphamide induced cystitis is an established model for the study of bladder injury and wound healing. Glycosylation is an important modification mechanism that regulates the structure and function of secreted proteins and growth factors from inflammation sites. We determined the effect of cyclophosphamide induced cystitis on O-GlcNAc mediated glycosylation in the bladder. Cystitis in WT C57BL6 mice was induced with intraperitoneal cyclophosphamide. Retrieved bladders were analyzed using histology, immunohistochemistry, reverse transcriptase-polymerase chain reaction and Western blot for glycosylation associated factors. Acute bladder injury was seen up to 168 hours (7 days) after injection. Reverse transcriptase-polymerase chain reaction revealed down-regulation of O-GlcNAc transferase, a key enzyme in O-GlcNAc mediated glycosylation, at the 8, 48 and 168-hour time points. Also, the glycosidase menangioma expressed antigen 5 was up-regulated at similar time points. Western blot analysis revealed decreased glycosylated protein during cyclophosphamide induced inflammation. To our knowledge we report the first study of alterations in O-GlcNAc mediated glycosylation activity in bladders with cyclophosphamide induced cystitis...Continue Reading

References

Feb 7, 2001·The Journal of Biological Chemistry·W A Lubas, J A Hanover
Jun 25, 2003·Glycoconjugate Journal·B J CampbellJ M Rhodes
Jun 3, 2005·Nature Reviews. Drug Discovery·Danielle H Dube, Carolyn R Bertozzi
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Dec 13, 2006·Genes & Development·Jennifer M Halbleib, W James Nelson
May 13, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Dongqi XingSuzanne Oparil
Jan 8, 2009·Disease Markers·Olga Gornik, Gordan Lauc

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Citations

Sep 19, 2015·Immunity & Ageing : I & a·Shiva MarthandanStephan Diekmann
Dec 19, 2013·Journal of Preventive Medicine and Public Health = Yebang Ŭihakhoe Chi·Jong Youn MoonYoon Hyung Park

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