Abstract
Cyclophosphamide induced cystitis is an established model for the study of bladder injury and wound healing. Glycosylation is an important modification mechanism that regulates the structure and function of secreted proteins and growth factors from inflammation sites. We determined the effect of cyclophosphamide induced cystitis on O-GlcNAc mediated glycosylation in the bladder. Cystitis in WT C57BL6 mice was induced with intraperitoneal cyclophosphamide. Retrieved bladders were analyzed using histology, immunohistochemistry, reverse transcriptase-polymerase chain reaction and Western blot for glycosylation associated factors. Acute bladder injury was seen up to 168 hours (7 days) after injection. Reverse transcriptase-polymerase chain reaction revealed down-regulation of O-GlcNAc transferase, a key enzyme in O-GlcNAc mediated glycosylation, at the 8, 48 and 168-hour time points. Also, the glycosidase menangioma expressed antigen 5 was up-regulated at similar time points. Western blot analysis revealed decreased glycosylated protein during cyclophosphamide induced inflammation. To our knowledge we report the first study of alterations in O-GlcNAc mediated glycosylation activity in bladders with cyclophosphamide induced cystitis...Continue Reading
References
Jan 1, 1984·Urologia Internationalis·E KunzeJ Pohl
Apr 1, 1994·British Journal of Pharmacology·A AhluwaliaS Giuliani
Feb 23, 2000·Experimental Neurology·M A Vizzard
Feb 7, 2001·The Journal of Biological Chemistry·W A Lubas, J A Hanover
Sep 22, 2001·Biochimica Et Biophysica Acta·C SlawsonR Potter
Aug 2, 2002·Cell·Xiaoyong YangJeffrey E Kudlow
Jun 25, 2003·Glycoconjugate Journal·B J CampbellJ M Rhodes
Sep 23, 2003·Cell Biology International·Kristijan JezernikDasa Koprivec
Jan 30, 2004·Molecular and Cellular Biology·Niall O'DonnellJamey D Marth
Nov 13, 2004·The Journal of Urology·Elaine MurrayMargaret A Vizzard
Jun 3, 2005·Nature Reviews. Drug Discovery·Danielle H Dube, Carolyn R Bertozzi
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Dec 13, 2006·Genes & Development·Jennifer M Halbleib, W James Nelson
Apr 27, 2007·Nature·Gerald W HartChad Slawson
May 22, 2007·Cancer·Tait D ShanafeltNeil E Kay
Mar 15, 2008·Journal of Pineal Research·Dasa ZupancicGaj Vidmar
May 13, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Dongqi XingSuzanne Oparil
Jul 12, 2008·EMBO Reports·Alexander Golks, Danilo Guerini
Jan 8, 2009·Disease Markers·Olga Gornik, Gordan Lauc