O-GlcNAc modification of radial glial vimentin filaments in the developing chick brain

Brain Cell Biology
Andrew M Farach, Deni S Galileo

Abstract

We examined the post-translational modification of intracellular proteins by beta-O-linked N-acetylglucosamine (O-GlcNAc) with regard to neurofilament phosphorylation in the developing chick optic tectum. A regulated developmental pattern of O-GlcNAcylation was discovered in the developing brain. Most notably, discernible staining occurs along radial glial filaments but not along neuronal filaments in vivo. Immunohistochemical analyses in sections of progressive stages of development suggest upregulation of O-GlcNAc in the ependyma, tectofugal neuron bodies, and radial glial processes, but not in axons. In contrast, double-label immunostaining of monolayer cultures made from dissociated embryonic day (E) 7 optic tecta revealed O-GlcNAcylation of most axons. Labeling of brain sections together with Western blot analyses showed O-GlcNAc modification of a few discrete proteins throughout development, and suggested vimentin as the protein in radial glia. Immunoprecipitation of vimentin from E9 whole brain lysates confirmed O-GlcNAcylation of vimentin in development. These results indicate a regulated pattern of O-GlcNAc modification of vimentin filaments, which in turn suggests a role for O-GlcNAc-modified intermediate filaments in...Continue Reading

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Citations

Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Jan 7, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Jui M Dave, Kayla J Bayless
Nov 18, 2014·Frontiers in Physiology·Caroline Cieniewski-BernardBruno Bastide
Oct 12, 2016·Glycoconjugate Journal·Li Hui WuXing Zhong Wu
Dec 23, 2017·Asian Pacific Journal of Tropical Medicine·Hua LuoMing-Ying Luo
Jan 11, 2019·The Journal of Biological Chemistry·Gerald W Hart
Aug 27, 2019·Critical Reviews in Clinical Laboratory Sciences·Brigitta ButtariRachele Riganò
Sep 8, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alison E PattesonPaul A Janmey

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