O-GlcNAcylation of kinases.

Biochemical and Biophysical Research Communications
Wagner B DiasGerald W Hart

Abstract

Recent evidence indicates that site-specific crosstalk between O-GlcNAcylation and phosphorylation and the O-GlcNAcylation of kinases play an important role in regulating cell signaling. However, relatively few kinases have been analyzed for O-GlcNAcylation. Here, we identify additional kinases that are substrates for O-GlcNAcylation using an in vitro OGT assay on a functional kinase array. Forty-two kinases were O-GlcNAcylated in vitro, representing 39% of the kinases on the array. In addition, we confirmed the in vivo O-GlcNAcylation of three identified kinases. Our results suggest that O-GlcNAcylation may directly regulate a substantial number of kinases and illustrates the increasingly complex relationship between O-GlcNAcylation and phosphorylation in cellular signaling.

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Citations

Apr 27, 2013·Cell Stress & Chaperones·Jennifer A GrovesNatasha E Zachara
Mar 7, 2014·Clinical Proteomics·Junfeng Ma, Gerald W Hart
Apr 3, 2014·Nucleic Acids Research·Qiuran XuHuadong Pei
Nov 26, 2015·Current Opinion in Chemical Biology·Cedric D MooreHeng Zhu
Apr 5, 2015·Cellular and Molecular Life Sciences : CMLS·Sujin ParkJin Won Cho
Jun 12, 2014·Frontiers in Oncology·Rafaela Muniz de QueirozWagner Barbosa Dias
Nov 27, 2014·Frontiers in Endocrinology·Parunya ChaiyawatVoraratt Champattanachai
Jun 26, 2015·Organic & Biomolecular Chemistry·Silviya R Stateva, Antonio Villalobo
Jan 13, 2018·Journal of Bioenergetics and Biomembranes·Andréia Vasconcelos-Dos-SantosWagner B Dias
Jun 7, 2018·The Journal of Biological Chemistry·Diogo de Barros PeruchettiCelso Caruso-Neves
May 3, 2018·The FEBS Journal·Saar A M van der LaarseAlbert J R Heck
Jan 11, 2019·The Journal of Biological Chemistry·Gerald W Hart
Oct 18, 2019·Traffic·Sadia RahmaniCostin N Antonescu
Oct 23, 2014·The Journal of Biological Chemistry·John Janetzko, Suzanne Walker
Jul 13, 2016·The Journal of Biological Chemistry·Rafaela Muniz de QueirozChad Slawson
Jun 3, 2016·Cold Spring Harbor Perspectives in Biology·Michael J Lee, Michael B Yaffe
Nov 6, 2018·Frontiers in Endocrinology·Gwendoline BourréCaroline Smet-Nocca
Oct 23, 2014·The Journal of Biological Chemistry·Brian A Lewis, John A Hanover
Aug 15, 2018·Frontiers in Endocrinology·Caifei Liu, Jing Li
May 1, 2020·Journal of Biomedical Science·Yi-Hsuan ChangKuo-I Lin
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yunfan YangXiaoyong Yang
Nov 14, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shamik DasLalita A Shevde
Jan 9, 2021·Chemical Reviews·Junfeng MaGerald W Hart
Mar 11, 2020·ACS Chemical Biology·Paul A Schwein, Christina M Woo
Jul 31, 2020·Physiological Reviews·John C ChathamAdam R Wende
Feb 19, 2021·Frontiers in Endocrinology·Aiyun YuanJing Li
Feb 8, 2021·Biology of Reproduction·Abigail M Maucieri, David H Townson
May 4, 2021·Frontiers in Chemistry·Matjaž WeissMarko Anderluh
Mar 4, 2014·Journal of the American Chemical Society·Rodrigo F Ortiz-MeozSuzanne Walker
May 24, 2016·Journal of Proteome Research·Junfeng MaGerald W Hart

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