O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae

Journal of Biochemistry
Hiroto HirayamaYoshifumi Jigami

Abstract

In Saccharomyces cerevisiae, protein O-mannosylation, which is executed by protein O-mannosyltransferases, is essential for a variety of biological processes as well as for conferring solubility to misfolded proteins. To determine if O-mannosylation plays an essential role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins, we used a model misfolded protein, Gas1*p. The O-mannose content of Gas1*p, which is transferred by protein O-mannosyltransferases, was higher than that of Gas1p. Both Pmt1p and Pmt2p, which do not transfer O-mannose to correctly folded Gas1p, participated in the O-mannosylation of Gas1*p. Furthermore, in a pmt1 Delta pmt2 Delta double-mutant background, degradation of Gas1*p is altered from a primarily proteasome dependent to a vacuolar protease-dependent pathway. This process is in a manner dependent on a Golgi-to-endosome sorting function of the VPS30 complex II. Collectively, our data suggest that O-mannosylation plays an important role for proteasome-dependent degradation of Gas1*p via the ERAD pathway and when O-mannosylation is insufficient, Gas1*p is degraded in the vacuole. Thus, we propose that O-mannosylation by Pmt1p and Pmt2p might be a key step in the targeting of some...Continue Reading

Citations

Feb 13, 2010·The Journal of Biological Chemistry·Hiroto HirayamaTadashi Suzuki
May 12, 2009·Glycobiology·Mark Lommel, Sabine Strahl
Feb 12, 2013·Genetics·Charles K Barlowe, Elizabeth A Miller
Jun 13, 2012·The Journal of Cell Biology·Eric M RubensteinMark Hochstrasser
Oct 16, 2015·Nature Reviews. Molecular Cell Biology·Chengchao Xu, Davis T W Ng
Mar 14, 2013·FEMS Microbiology Reviews·Marizela DelicBrigitte Gasser
Jun 22, 2016·The Journal of Cell Biology·Natalia SikorskaVeit Goder
Apr 30, 2016·Molecular & Cellular Proteomics : MCP·Thibaut LégerJean-Michel Camadro
Nov 13, 2010·Circulation Research·Jody GroenendykMarek Michalak
Jan 28, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akiko Murakami-SekimataAkihiko Nakano
Feb 13, 2014·The Journal of Biological Chemistry·Martin LoiblSabine Strahl
Dec 20, 2017·The Journal of Cell Biology·Yi-Shi LiuMorihisa Fujita
Jan 15, 2011·Molecular Microbiology·Javier ArroyoSabine Strahl
Dec 15, 2019·International Journal of Molecular Sciences·Joan Castells-BallesterSabine Strahl
Oct 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joan Castells-BallesterSabine Strahl
Sep 25, 2020·The Journal of Biological Chemistry·Xin-Yu GuoMorihisa Fujita
Dec 15, 2020·Biochimica Et Biophysica Acta. General Subjects·Satoshi NinagawaKazutoshi Mori
Jan 27, 2021·International Journal of Molecular Sciences·Kunio Nakatsukasa
Dec 15, 2010·Journal of Cell Science·Veit Goder, Alejandro Melero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.