O2 deprivation induces a major depolarization in brain stem neurons in the adult but not in the neonatal rat

The Journal of Physiology
G G Haddad, D F Donnelly

Abstract

1. In order to study the neuronal response to hypoxia with maturation, hypoglossal neurons from adult and neonatal rat (3-7, 14-16, 21 and 28 days) brain stem slices were subjected to O2 deprivation. All neurons depolarized and showed no evidence of hyperpolarization at any time during the hypoxic period. 2. The magnitude of depolarization was about three-fold larger in adult hypoglossal neurons (mean = 32.0 mV) than in young neonatal neurons (mean = 10.4-11.2 mV) during hypoxic exposure (15-20 Torr) of 5 min. During longer periods of hypoxia of 15-30 min, neonatal cells showed an increase in the magnitude of depolarization reaching a level close to 80% of that in the adult. 3. In the early phase of hypoxia, adult neurons increased peak and steady-state spike frequency to induced current injections. Later, both spike frequencies decreased and, in 1/2 of adult neurons, there was a depolarization block. Input resistance (RN) of most adult neurons increased during hypoxia (RN = 180% of control after 5 min). Though neonatal neurons increased firing frequency, none had depolarization block and there was no increase in RN. 4. Tetrodotoxin (TTX), tetraethylammonium (TEA), apamin, high Mg2+/low Ca2+ solutions and intracellular ethylene...Continue Reading

Citations

May 23, 1998·The Journal of Physiology·S L MironovD W Richter
Jul 1, 1991·Irish Journal of Medical Science·C S Breathnach
Jan 22, 1996·Brain Research. Developmental Brain Research·P C Nolan, T G Waldrop
Dec 23, 1996·Brain Research. Developmental Brain Research·J D MarksG G Haddad
Jan 1, 1994·Experimental Brain Research·C JiangG G Haddad
Sep 17, 2002·Neuroscience Letters·Yoshiyuki SakataShoji Nakamura
Feb 11, 1998·The Journal of Physiology·O PierreficheK M Spyer
Sep 8, 1998·The Journal of Physiology·S L Mironov, D W Richter
Oct 17, 2002·The Journal of Physiology·Olivier PascualJean Champagnat
Nov 18, 2000·The Journal of Physiology·A K Hammarström, P W Gage
Mar 31, 1998·The Journal of Physiology·J M RamirezD W Richter
Nov 17, 2001·The Journal of Physiology·M HallerD W Richter
Mar 11, 2000·The European Journal of Neuroscience·S L MironovD W Richter
Mar 4, 2008·Neuropharmacology·Kresimir Krnjević
Oct 11, 2003·Journal of Neurochemistry·Shane E MunnsPeter G Arthur
Sep 10, 2014·Journal of Neuroscience Methods·Jason S Nasse
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·G G HaddadT Xu
May 15, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A Sawczuk, K M Mosier
Dec 16, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R C TaskerS R Williams
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R C TaskerS R Williams
Sep 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P E BicklerB M Hansen
Jun 14, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Philip E BicklerLeslie T Buck
Apr 15, 1993·The Journal of Comparative Neurology·Y Xia, G G Haddad

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