O2 regulates surfactant protein A mRNA transcription and stability in human fetal lung in vitro

The American Journal of Physiology
M J AcarreguiJeanne M Snyder

Abstract

The effect of O2 on surfactant protein (SP) A mRNA transcription and half-life was determined in midtrimester human fetal lung tissue cultured in either 20 (control) or 70% O2. Incubation of tissues in 70% O2 resulted in a 133% increase in SP-A mRNA transcription rate compared with control tissues. The SP-A mRNA half-life was increased by 54% in lung tissues cultured in 70% O2 vs. control tissues. Western blot analysis indicated a threefold increase in SP-A in the 70% O2 condition, demonstrating that O2 regulation of SP-A mRNA levels results in corresponding changes in SP-A levels. Primer extension assays were performed to determine whether the observed increase in SP-A mRNA levels is secondary to the preferential expression of one of the human SP-A genes, SP-A1 or SP-A2. Transcripts of both the SP-A1 and SP-A2 genes were increased approximately 100% in tissues maintained in 70% O2 compared with control tissues. These data demonstrate that O2 regulates human SP-A mRNA levels by both transcriptional and posttranscriptional mechanisms. Furthermore, because there is no differential effect of O2 on the expression of SP-A1 vs. SP-A2 mRNA, the properties of these genes that mediate regulation by O2 must be conserved between the two g...Continue Reading

References

Apr 1, 1992·American Journal of Respiratory Cell and Molecular Biology·S L KatyalJ Locker
Apr 1, 1992·Journal of Molecular and Cellular Cardiology·D B CowanD A Mickle
Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·L M NogeeJ A Whitsett
Jan 1, 1981·Cell and Tissue Research·J M SnyderC R Mendelson
Jul 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A M ChoiN J Holbrook
Jan 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A M Karinch, J Floros
Feb 1, 1993·The Journal of Clinical Investigation·L B Clerch, D Massaro
Dec 1, 1995·The Journal of Clinical Investigation·J K LiaoP M Hassoun
Mar 1, 1996·The European Respiratory Journal·Z L HarrisJ I Sznajder
May 1, 1997·Pediatric Research·T N George, J M Snyder

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Citations

Jun 14, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sarabjit S PhokelaJoseph L Alcorn
Jan 10, 2004·Antioxidants & Redox Signaling·Vijay Boggaram

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