OASTL-A1 functions as a cytosolic cysteine synthase and affects arsenic tolerance in rice.

Journal of Experimental Botany
Chengcheng WangFang-Jie Zhao

Abstract

Arsenic (As) contamination in paddy soil can cause phytotoxicity and elevated As accumulation in rice grains. Arsenic detoxification is closely linked to sulfur assimilation, but the genes involved have not been described in rice. In this study, we characterize the function of OASTL-A1, an O-acetylserine(thiol) lyase, in cysteine biosynthesis and detoxification of As in rice. Tissue expression analysis revealed that OsOASTL-A1 is mainly expressed in roots at the vegetative growth stage and in nodes at the reproductive stage. Furthermore, the expression of OsOASTL-A1 in roots was strongly induced by As exposure. Transgenic rice plants expressing pOsOASTL-A1::GUS (β-glucuronidase) indicated that OsOASTL-A1 was strongly expressed in the outer cortex and the vascular cylinder in the root mature zone. Subcellular localization using OsOASTL-A1:eGFP (enhanced green fluorescent protein) fusion protein showed that OsOASTL-A1 was localized to the cytosol. In vivo and in vitro enzyme activity assays showed that OsOASTL-A1 possessed the O-acetylserine(thiol) lyase activity. Knockout of OsOASTL-A1 led to significantly lower levels of cysteine, glutathione, and phytochelatins in roots and increased sensitivity to arsenate stress. Furthermore...Continue Reading

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Citations

Jan 9, 2021·Journal of Hazardous Materials·Amos Musyoki MawiaPeisong Hu
Nov 15, 2020·Journal of Hazardous Materials·Fenglin DengZhong-Hua Chen
Mar 4, 2021·Nature Communications·Sheng-Kai SunFang-Jie Zhao

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