Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice

Endocrinology
Fang XieJennifer R Wood

Abstract

RNAs stored in the metaphase II-arrested oocyte play important roles in successful embryonic development. Their abundance is defined by transcriptional activity during oocyte growth and selective degradation of transcripts during LH-induced oocyte maturation. Our previous studies demonstrated that mRNA abundance is increased in mature ovulated oocytes collected from obese humans and mice and therefore may contribute to reduced oocyte developmental competence associated with metabolic dysfunction. In the current study mouse models of diet-induced obesity were used to determine whether obesity-dependent increases in proinflammatory signaling regulate ovarian abundance of oocyte-specific mRNAs. The abundance of oocyte-specific Bnc1, Dppa3, and Pou5f1 mRNAs as well as markers of proinflammatory signaling were significantly increased in ovaries of obese compared with lean mice which were depleted of fully grown preovulatory follicles. Chromatin-immunoprecipitation analyses also demonstrated increased association of phosphorylated signal transducer and activator of transcription 3 with the Pou5f1 promoter in ovaries of obese mice suggesting that proinflammatory signaling regulates transcription of this gene in the oocyte. The cecum m...Continue Reading

References

Jan 1, 1980·Developmental Biology·R Bachvarova, V De Leon
Apr 27, 2000·Molecular Immunology·K Imada, W J Leonard
Aug 5, 2000·Applied and Environmental Microbiology·I DahllöfS Kjelleberg
Dec 5, 2003·Current Biology : CB·Bernhard PayerM Azim Surani
Apr 10, 2004·Human Reproduction Update·Ruijin WuRebecca L Robker
Aug 25, 2004·Science·Aleksandar RajkovicMartin M Matzuk
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Mar 8, 2005·Gene Expression Patterns : GEP·Katherine MurphyMelissa Pepling
Feb 18, 2006·Science·Antonio J GiraldezAlexander F Schier
Apr 21, 2006·Development·Jun MaHung Tseng
Jun 6, 2006·Journal of Molecular Evolution·Jason EvansJames Foster
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Sep 26, 2006·The Journal of Biological Chemistry·Youngsok Choi, Aleksandar Rajkovic
Dec 5, 2006·Nature Cell Biology·Toshinobu NakamuraToru Nakano
Dec 7, 2006·The Journal of Clinical Endocrinology and Metabolism·Jennifer R WoodJerome F Strauss
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Dec 22, 2006·Nature·Peter J TurnbaughJeffrey I Gordon
Apr 26, 2007·Diabetes·Patrice D CaniRémy Burcelin
May 15, 2007·Biology of Reproduction·Youngsok ChoiAleksandar Rajkovic
Jun 15, 2007·BMC Developmental Biology·Saurabh JoshiJurrien Dean
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel N FrankNorman R Pace
Feb 20, 2008·Genes & Development·Dana N LevasseurStuart H Orkin
Oct 8, 2008·BMC Developmental Biology·Maurizio ZuccottiJames Adjaye
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Nov 21, 2008·Molecular Human Reproduction·Jing-He TanJie Zhang
Dec 2, 2008·Nature·Peter J TurnbaughJeffrey I Gordon
Dec 19, 2009·Cytokine & Growth Factor Reviews·Sergei I Grivennikov, Michael Karin
Feb 25, 2010·Development·Lei LiJurrien Dean
Mar 2, 2010·Human Reproduction·Inna V LandresRuth B Lathi
Apr 7, 2010·The Journal of Clinical Investigation·Roger Gosden, Bora Lee
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
May 29, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claire Barbier de La SerreHelen E Raybould
Jun 22, 2010·The Journal of Reproduction and Development·Ken-Ichiro ABEFugaku AOKI
Sep 10, 2010·The ISME Journal·Catherine LozuponeRob Knight
Sep 21, 2010·Applied and Environmental Microbiology·S C FernandoU Desilva
Feb 22, 2011·Journal of Reproductive Immunology·Rebecca L RobkerXing Yang

❮ Previous
Next ❯

Citations

Dec 9, 2016·Physiology·Kathleen JaegerKelle H Moley
Nov 8, 2018·Endocrinology·Natalie M HohosMalgorzata E Skaznik-Wikiel
Apr 24, 2019·Human Fertility : Journal of the British Fertility Society·Einat Haikin HerzbergerAmir Wiser
Apr 23, 2021·PloS One·Sathish Kumar NatarajanAnn Anderson-Berry
Dec 18, 2021·Biology of Reproduction·Macarena B GonzalezRyan D Rose

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.