Obesity increases airway smooth muscle responses to contractile agonists

American Journal of Physiology. Lung Cellular and Molecular Physiology
Sarah OrfanosReynold A Panettieri

Abstract

The asthma-obesity syndrome represents a major public health concern that disproportionately contributes to asthma severity and induces insensitivity to therapy. To date, no study has shown an intrinsic difference between human airway smooth muscle (HASM) cells derived from nonobese subjects and those derived from obese subjects. The objective of this study was to address whether there is a greater response to agonist-induced calcium mobilization, phosphorylation of myosin light chain (MLC), and greater shortening in HASM cells derived from obese subjects. HASM cells derived from nonobese and obese subjects were age and sex matched. Phosphorylation of MLC was measured after having been stimulated by carbachol. Carbachol- or histamine-induced mobilization of calcium and cell shortening were assessed in HASM cells derived from nonobese and obese donors. Agonist-induced MLC phosphorylation, mobilization of calcium, and cell shortening were greater in obese compared with non-obese-derived HASM cells. The MLC response was comparable in HASM cells derived from obese nonasthma and nonobese fatal asthma subjects. HASM cells derived from obese female subjects were more responsive to carbachol than HASM cells derived from obese male subj...Continue Reading

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Citations

Sep 6, 2019·Current Allergy and Asthma Reports·Peng Zhang, Joe Zein
Aug 24, 2019·British Journal of Pharmacology·James R DochertyAmrita Ahluwalia
Nov 28, 2019·Annals of the American Thoracic Society·Deepa Rastogi
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Oct 8, 2020·International Journal of Molecular Sciences·Weronika BanachDamian Skrypnik
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Dec 19, 2021·Current Allergy and Asthma Reports·Varun Sharma, Douglas C Cowan

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Methods Mentioned

BETA
Assay

Software Mentioned

GraphPad Prism
MATLAB
Graphad Prism

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