Obesity-induced tissue free radical generation: an in vivo immuno-spin trapping study.

Free Radical Biology & Medicine
Nicholas K H KhooEric E Kelley

Abstract

Assessment of tissue free radical production is routinely accomplished by measuring secondary by-products of redox reactions and/or diminution of key antioxidants such as reduced thiols. However, immuno-spin trapping, a newly developed immunohistochemical technique for detection of free radical formation, is garnering considerable interest as it allows for the visualization of 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-adducted molecules. Yet, to date, immuno-spin trapping reports have utilized in vivo models in which successful detection of free radical adducts required exposure to lethal levels of oxidative stress not reflective of chronic inflammatory disease. To study the extents and anatomic locations of more clinically relevant levels of radical formation, we examined tissues from high-fat (HF) diet-fed mice, a model of low-grade chronic inflammation known to demonstrate enhanced rates of reactive species production. Mice subjected to 20 weeks of HF diet displayed increased free radical formation (anti-DMPO mean fluorescence staining) in skeletal muscle (0.863±0.06 units vs 0.512±0.07 units), kidney (0.076±0.0036 vs 0.043±0.0025), and liver (0.275±0.012 vs 0.135±0.014) compared to control mice fed normal laboratory chow (NC)...Continue Reading

References

Oct 20, 1998·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·A E Schultze, R A Roth
Feb 6, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Margaret M TarpeyMatthew B Grisham
Jan 12, 2008·The Journal of Clinical Investigation·Charlotte BonnardJennifer Rieusset
Jan 19, 2008·American Journal of Respiratory and Critical Care Medicine·Ari L ZaimanRubin M Tuder
Apr 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Patricia CassinaRafael Radi
Jun 19, 2008·American Journal of Physiology. Endocrinology and Metabolism·Krisztian StadlerMaria B Kadiiska
Nov 26, 2009·Journal of the National Cancer Institute·Chikara KojimaMichael P Waalkes
Jan 12, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Everaldo M RedoutWarner S Simonides
Mar 10, 2011·American Journal of Physiology. Endocrinology and Metabolism·Christine RuggieroKrisztian Stadler
Jul 2, 2011·Kidney International·Yasuhiko TominoTomohito Gohda
Sep 2, 2011·American Journal of Physiology. Renal Physiology·Alex Odermatt
Oct 27, 2011·Free Radical Biology & Medicine·Balaraman KalyanaramanHarry Ischiropoulos
Dec 6, 2011·The Journal of Biological Chemistry·Jacek ZielonkaBalaraman Kalyanaraman

❮ Previous
Next ❯

Citations

Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Adrienne L KingDavid J Lefer
Dec 3, 2014·Free Radical Biology & Medicine·Yuya HorinouchiRonald P Mason
Oct 25, 2016·Journal of Neurochemistry·Cameron DezfulianHulya Bayir
May 24, 2013·International Journal of Molecular Sciences·Isabella SaviniLuciana Avigliano
Apr 2, 2015·EMBO Molecular Medicine·Marion SeillierAlice Carrier
Aug 9, 2018·American Journal of Physiology. Endocrinology and Metabolism·Daniel B HarmonRobert M O'Doherty
Oct 2, 2015·Current Protocols in Cytometry·Nicholas K H KhooEric E Kelley
Sep 27, 2018·International Wound Journal·James A D BerryMargaret R S Wacker
Apr 10, 2019·Scientific Reports·Wu-Qing HuangCai-Xia Zhang
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Bartosz ProniewskiStefan Chlopicki
Nov 7, 2015·Arthritis Research & Therapy·Satoshi SuzukiMorio Matsumoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Diseases: Risk Factors

Cardiovascular disease is a significant health concern. Risk factors include hypertension, obesity, dyslipidemia and smoking. Women who are postmenopausal are at an increased risk of heart disease. Here is the latest research for risk factors of cardiovascular disease.