Obesity-related CpG Methylation (cg07814318) of Kruppel-like Factor-13 (KLF13) Gene with Childhood Obesity and its cis-Methylation Quantitative Loci

Scientific Reports
In-Uk KohSuman Lee

Abstract

The cg07814318 hypermethylation of Kruppel-like factor 13 (KLF13) gene has been reported for its relevancy with Body Mass Index (BMI) from European origin. We explored the cg07814318 methylation and its cis-meQTL (cis-methylation quantitative loci) of KLF13 from a childhood obesity cohort. The cg07814318 methylation in blood was significantly associated with obesity and correlated with several obesity-related physical and biochemical traits. We examined the same loci from purified three human cell types (n = 47), i.e., pre-adipocytes, adipocytes and islets. The cg07814318 methylation pattern in pre-adipocytes and islets were significant higher in cells from subjects with a higher BMI compared with control subjects. By exome sequencing of KLF13 gene in blood with the same cohort, we found nine SNPs (single nucleotide polymorphisms) within its gene body, and two SNPs (rs11537749 and rs12595641) were as cis-meQTL of cg07814318. There was the 2.01% methylation change of cg07814318 between homozygous dominant and recessive genotypes, especially, in rs12595641. The sequencing variations within KLF13 genes could drive dynamic modifications of obesity-related CpG methylation. Differential DNA methylation patterns in the KLF13 gene dete...Continue Reading

References

Jun 28, 2006·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S YooE Sung
Dec 15, 2006·Nature·Jean-Pierre Després, Isabelle Lemieux
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meixia ZhouAlan M Krensky
Oct 9, 2009·Nature·Teri A ManolioPeter M Visscher
May 11, 2010·Lancet·Joan C HanSue Y S Kimm
Dec 2, 2010·Pacific Symposium on Biocomputing·Francisco M DE LA VegaSuzanne M Leal
Mar 19, 2014·Lancet·Katherine J DickNilesh J Samani
Apr 11, 2014·International Journal of Endocrinology·Kevin J BasileStruan F A Grant
May 14, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seok Joo KwonCarol Clayberger
Feb 13, 2015·Nature·Adam E LockeElizabeth K Speliotes
Jun 19, 2015·Cell & Bioscience·Shuzhong JiangSiwen Jiang

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Methods Mentioned

BETA
exome sequencing
PCR
pancreatectomy

Software Mentioned

R scripts
UCSE
PLINK
IlluminaBeadstudio

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