Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

Micron : the International Research and Review Journal for Microscopy
Gi-Ja LeeHun-Kuk Park

Abstract

Glomerular mesangial cells (MCs) are centrally located in the glomerulus. MCs control not only glomerular filtration, but also the response to local injury, including cell proliferation and basement membrane remodeling. Angiotensin II (Ang II) plays an important role in kidney function regulation, and participates in the progression of renal damage, as well as mesangial injury. However, studies on Ang II effects on MCs have used indirect methods, such as gene and protein expression after MC injury. In this study, we visually observed structural and mechanical changes to MC after Ang II treatment using atomic force microscopy (AFM). We obtained AFM topography and deflection images of live MCs, as well as fixed MCs in liquid, before and after Ang II treatment. Real-time imaging showed the dynamic movement of live MCs induced by Ang II. Changes in MC elastic property after Ang II treatment were measured using force-distance curves. AFM images of fixed and live MCs showed that cells contracted after Ang II exposure, with the nucleus height increasing within 20 min of Ang II stimulation. Force-distance analysis showed that Ang II caused MCs to stiffen (p < 0.0001). In conclusion, we demonstrated that AFM is an effective tool for rea...Continue Reading

References

Oct 1, 1991·Kidney International·M KitamuraO Sakai
Jan 15, 1998·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·S Klahr, J Morrissey
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Sep 22, 2001·Hypertension·S A MezzanoJ Egido
Apr 21, 2005·Kidney International·Hans Oberleithner
Nov 11, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marta Ruiz-OrtegaJesús Egido
Jun 28, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Li-Ping XuShaowei Chen

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Citations

Apr 16, 2013·Micron : the International Research and Review Journal for Microscopy·Young Ju LeeHun-Kuk Park
Oct 21, 2011·The Angle Orthodontist·Sung-Hwan ChoiChung-Ju Hwang
Jul 22, 2010·Micron : the International Research and Review Journal for Microscopy·Gi-Ja LeeHun-Kuk Park
Nov 6, 2010·Micron : the International Research and Review Journal for Microscopy·Gi-Ja LeeHun-Kuk Park
Jun 15, 2011·Journal of Microbiological Methods·Hayden K WebbElena P Ivanova
Mar 20, 2013·Electrolyte & Blood Pressure : E & BP·Kyung Hwan Jeong, Sang Ho Lee

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