Observation of antibacterial effect of biodegradable polymeric nanoparticles on Staphylococcus epidermidis biofilm using FE-SEM with an ionic liquid

Microscopy
Chisato TakahashiHiromitsu Yamamoto

Abstract

We successfully visualized the antibacterial behavior of biodegradable polymeric nanoparticles (NPs) on a biofilm formed by Staphylococcus epidermidis using field emission scanning electron microscopy (FE-SEM). A hydrophilic ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), was applied for observation using FE-SEM. The differences in adherence and penetration behavior of three types of NPs were revealed using this method and confocal laser scanning microscopy. Biodegradable poly(dl-lactide-co-glycolide) (PLGA) NPs were prepared by the emulsion solvent diffusion method. In this study, we treated the biofilm with three PLGA NPs: unmodified PLGA, PLGA modified with chitosan (CS) and clarithromycin (CAM)-loaded + CS-modified PLGA. The viability of S. epidermidis cells treated with PLGA NPs was estimated using the LIVE/DEAD BacLight kit to understand the antibacterial ability of each NP sample in a quantitative way. The results confirmed that CAM-loaded + CS-modified PLGA had high antibacterial ability on the biofilm. This novel observation technique would be useful in the development of drug formations and medical agents.

References

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Dec 30, 2014·Analytical and Bioanalytical Chemistry·Chisato TakahashiHiromitsu Yamamoto

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Citations

Apr 19, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Chisato TakahashiHiromitsu Yamamoto
Apr 28, 2016·Medical Molecular Morphology·Chisato TakahashiHiromitsu Yamamoto
May 23, 2017·Therapeutic Delivery·Tânia Santos de AlmeidaCatarina Pinto Reis
Jun 22, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Chisato TakahashiHiromitsu Yamamoto
Apr 8, 2020·Microscopy·Tetsuya Tsuda, Susumu Kuwabata
Jun 5, 2020·Future Microbiology·Gratiela G Pircalabioru, Mariana-Carmen Chifiriuc
Sep 13, 2020·Chembiochem : a European Journal of Chemical Biology·Bryan E J LeeKathryn Grandfield
Apr 13, 2021·Advanced Drug Delivery Reviews·Stine Egebro BirkLine Hagner Nielsen

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