Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

Nature Communications
Minjung SonGabriela S Schlau-Cohen

Abstract

Plants prevent photodamage under high light by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics to the protein structure. The mechanisms of dissipation remain debated, largely due to two challenges. First, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Second, experiments have been performed in detergent, which can induce non-native conformations, or in vivo, where contributions from homologous antenna complexes cannot be disentangled. Here, we overcome both challenges by applying ultrabroadband two-dimensional electronic spectroscopy to the principal antenna complex, LHCII, in a near-native membrane. Our data provide evidence that the membrane enhances two dissipative pathways, one of which is a previously uncharacterized chlorophyll-to-carotenoid energy transfer. Our results highlight the sensitivity of the photophysics to local environment, which may control the balance between light harvesting and dissipation in vivo.

References

Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ying-Zhong MaGraham R Fleming
Apr 15, 2004·Chemical Reviews·Tomás Polívka, Villy Sundström
Nov 23, 2007·Nature·Alexander V RubanRienk van Grondelle
May 7, 2009·Photosynthesis Research·Gyozo Garab, Herbert van Amerongen
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stefan BodePeter J Walla
Oct 28, 2009·The Journal of Physical Chemistry. B·Gabriela S Schlau-CohenGraham R Fleming
Nov 12, 2009·Methods in Enzymology·T K RitchieS G Sligar
Feb 4, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marc G MüllerAlfred R Holzwarth
Jun 15, 2010·Physical Chemistry Chemical Physics : PCCP·Akihito IshizakiGraham R Fleming
Jun 17, 2010·Biophysical Journal·Tjaart P J KrügerRienk van Grondelle
May 17, 2011·Biochimica Et Biophysica Acta·Alexander V RubanChristopher D P Duffy
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tjaart P J KrügerRienk van Grondelle
Sep 24, 2011·Nature Chemistry·Gregory D ScholesRienk van Grondelle
Nov 22, 2011·Biophysical Journal·Anjali PanditWillem J de Grip
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Laura WilkWerner Kühlbrandt
Apr 16, 2013·Current Opinion in Plant Biology·Krishna K Niyogi, Thuy B Truong
Sep 1, 1994·Photosynthesis Research·H A FrankM R Wasielewski
May 9, 2014·Physical Chemistry Chemical Physics : PCCP·Kym L WellsHowe-Siang Tan
Dec 20, 2014·The Journal of Biological Chemistry·Parveen AkhtarPetar H Lambrev
Feb 24, 2015·Nature Chemical Biology·Hristina StalevaRoman Sobotka
May 29, 2015·Physical Chemistry Chemical Physics : PCCP·Jevgenij ChmeliovChristopher D P Duffy
Aug 12, 2015·The Journal of Physical Chemistry Letters·Gabriela S Schlau-CohenW E Moerner
Jun 1, 2016·Nature Plants·Jevgenij ChmeliovLeonas Valkunas
Oct 31, 2016·Biochimica Et Biophysica Acta. Biomembranes·Emanuela Crisafi, Anjali Pandit
Feb 9, 2017·Chemical Reviews·Ilia G Denisov, Stephen G Sligar
Aug 17, 2017·Physical Chemistry Chemical Physics : PCCP·Kieran F FoxChristopher D P Duffy

❮ Previous
Next ❯

Citations

Jun 23, 2020·Journal of Experimental Botany·Matthew P Johnson
Aug 21, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Francesco SacconAlexander V Ruban
Aug 21, 2020·Chemical Communications : Chem Comm·Vangelis DaskalakisTaxiarchis Stergiannakos
Nov 28, 2020·Nature Communications·Eric A ArsenaultGraham R Fleming
Dec 11, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Sanjay J DhobleO P Pandey
Aug 21, 2020·IScience·Francesco SacconTomáš Polívka
Jan 26, 2021·The Journal of Biological Chemistry·Fei LiBruno Robert
Mar 25, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Minjung SonGabriela S Schlau-Cohen
Mar 24, 2021·The Journal of Physical Chemistry Letters·Václav ŠebelíkTomáš Polívka
Mar 26, 2021·The Journal of Physical Chemistry. B·Alexandra TrempeValter Zazubovich
Apr 30, 2021·The Plant Journal : for Cell and Molecular Biology·Renata WelcWieslaw I Gruszecki
Jun 19, 2021·Annual Review of Plant Biology·Roberto Bassi, Luca Dall'Osto
Jul 3, 2021·Biophysical Journal·Premashis MannaGabriela S Schlau-Cohen
Aug 19, 2020·The Journal of Physical Chemistry Letters·Michael S ChenThomas E Markland
Jul 30, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Margherita LapilloBenedetta Mennucci

❮ Previous
Next ❯

Methods Mentioned

BETA
light harvesting

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.