Observation of endoplasmic reticulum tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells

Biointerphases
Corryn E ChiniMary L Kraft

Abstract

Advances in three-dimensional secondary ion mass spectrometry (SIMS) imaging have enabled visualizing the subcellular distributions of various lipid species within individual cells. However, the difficulty of locating organelles using SIMS limits efforts to study their lipid compositions. Here, the authors have assessed whether endoplasmic reticulum (ER)-Tracker Blue White DPX®, which is a commercially available stain for visualizing the endoplasmic reticulum using fluorescence microscopy, produces distinctive ions that can be used to locate the endoplasmic reticulum using SIMS. Time-of-flight-SIMS tandem mass spectrometry (MS2) imaging was used to identify positively and negatively charged ions produced by the ER-Tracker stain. Then, these ions were used to localize the stain and thus the endoplasmic reticulum, within individual human embryonic kidney cells that contained higher numbers of endoplasmic reticulum-plasma membrane junctions on their surfaces. By performing MS2 imaging of selected ions in parallel with the precursor ion (MS1) imaging, the authors detected a chemical interference native to the cell at the same nominal mass as the pentafluorophenyl fragment from the ER-Tracker stain. Nonetheless, the fluorine seconda...Continue Reading

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Citations

Oct 20, 2018·The Analyst·Lei YinJingkai Gu
Feb 1, 2020·Rapid Communications in Mass Spectrometry : RCM·Abanoub MikhaelJoseph Banoub
Aug 20, 2019·Journal of Molecular Biology·Joan Bestard-EscalasGwendolyn Barceló-Coblijn
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Jun 9, 2020·ACS Nano·Brittney L GormanMary L Kraft
Jun 28, 2018·Journal of the American Society for Mass Spectrometry·Anne L BruinenRon M A Heeren
Aug 5, 2021·Biointerphases·Brittney L GormanMary L Kraft

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