Observation of erythrocyte dynamics in the retinal capillaries and choriocapillaris using ICG-loaded erythrocyte ghost cells

Investigative Ophthalmology & Visual Science
Robert FlowerIgnacy Gryczynski

Abstract

To find evidence of retinal vasomotion and to examine the relationship between erythrocyte dynamics and previously observed high-frequency pulsatile blood flow through the choriocapillaris. An osmotic shock technique was used to encapsulate indocyanine green (ICG) dye in erythrocyte ghost cells at a concentration that produced maximum cell fluorescence. By obviating the plasma staining that results from aqueous ICG's high affinity for plasma proteins, high contrast was maintained between reinjected ICG-loaded erythrocytes and their plasma background. High-speed, high-magnification ICG angiograms showing individual cell movement were recorded from the intact eyes of four monkeys and three rabbits for periods up to 30 seconds. In monkey retinal perifoveal capillaries, numerous erythrocytes were seen to pause for as long as 20 seconds and then resume transit. Similar pausing behavior was observed in the subfoveal choriocapillaris. Individual erythrocytes also were seen to pause in the rabbits' choriocapillaries below the medullary rays, where visualization of the cells was uninhibited by overlying retinal vasculature or dense pigment. Reinjected ICG-loaded erythrocytes permit routine visualization of retinal capillary and chorioca...Continue Reading

Citations

Mar 10, 2011·Nanomedicine·Liraz Larush, Shlomo Magdassi
Feb 20, 2014·Expert Opinion on Drug Delivery·Mauro Magnani, Luigia Rossi
Jan 25, 2014·Expert Opinion on Drug Delivery·Abdolhossein ZarrinMehrdad Hamidi
Jun 7, 2011·Blood Cells, Molecules & Diseases·Mark A MilanickKenith Meissner
Nov 30, 2011·Photodiagnosis and Photodynamic Therapy·Hyun-Ju Lim, Chung-Hun Oh
Mar 30, 2011·Experimental Eye Research·C StrohmaierH A Reitsamer
Jun 9, 2016·The British Journal of Ophthalmology·Francesco PichiSunil K Srivastava
Apr 7, 2017·Journal of Biomedical Optics·Sandra C Bustamante López, Kenith E Meissner
Jan 25, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Francesca PierigèMauro Magnani
Aug 23, 2020·Nutrients·Paolino NinfaliEmanuele Salvatore Scarpa
Dec 29, 2019·Scientific Reports·Breanna M TraceyOsamah J Saeedi
Sep 15, 2020·Therapeutic Advances in Ophthalmology·Samuel AsanadOsamah J Saeedi
Jul 31, 2019·Biomedical Optics Express·Dongyi WangOsamah Saeedi
Dec 15, 2016·Nanotechnology·Joshua M BurnsBahman Anvari
Oct 22, 2020·Vision·Gideon NkrumahJay Chhablani
Jun 3, 2021·Biomolecules·Taylor HanleyBahman Anvari
Apr 1, 2021·Annual Review of Biomedical Engineering·Jacob S BrennerVladimir R Muzykantov
Jul 23, 2021·Progress in Retinal and Eye Research·Raphael LejoyeuxJay Chhablani

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