PMID: 11926072Apr 3, 2002Paper

Observation of polarity induction by cytochemical localization of phenylalkylamine-binding sites in regenerating protoplasts of the moss Physcomitrella patens

Protoplasma
S C BhatlaR Reski

Abstract

Different external (e.g., light) and internal (e.g., auxin and calcium gradients) factors control differentiation of the moss protonema. The present investigations demonstrate that exogenously applied auxin, the pharmacological blockade of auxin efflux by naphthylphthalamic acid, and treatment with (-)bepridil, a calcium channel antagonist, inhibit protoplast division without affecting protoplast viability in the moss Physcomitrella patens. A fluorescently labelled phenylalkylamine (DM-Bodipy PAA), another calcium channel antagonist, was used as a probe for in vivo labelling of phenylalkylamine(PAA)-binding sites. The specificity of this binding was demonstrated by competition with (-)bepridil. Confocal laser scanning microscopy visualized PAA-binding sites on the plasma membrane and along the nuclear membrane as uniformly distributed clusters. During asymmetric division of P. patens protoplasts, however, fluorescence labelling particularly increases at the membrane invagination and later along the plate separating the new cells. Intracellular localization of PAA-binding sites, probably at the membranes of vesicles and vacuoles, significantly increases in the smaller daughter cell, destined to later form a polar outgrowth, the ...Continue Reading

Citations

Oct 3, 2009·Plant Cell Reports·Kishore C S PanigrahiMan Mohan Johri
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Enas QudeimatWolfgang Frank
May 4, 2006·Annual Review of Plant Biology·David CoveRalph Quatrano
Nov 7, 2012·Journal of Integrative Plant Biology·Beatrix ZabanPeter Nick
Nov 30, 2012·Biopolymers·Antônio Américo Barbosa VianaMaria Fátima Grossi-de-Sá
Jun 17, 2003·Journal of Plant Physiology·Satish Chander BhatlaElmar Hartmann
May 23, 2018·International Journal of Molecular Sciences·Kjell De VrieseSteffen Vanneste

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