PMID: 2525545Jun 1, 1989Paper

Observation of production of immunoactive prolactin by normal human connective tissue in cell culture

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
J ChapitisD H Riddick

Abstract

Data from our in vitro studies indicate a new source of prolactin (PRL)-like activity, normal human connective tissue. Fascial cells from primary culture and subsequent passages produced an extracellular antigen which specifically reacted in a radioimmunoassay RIA developed to detect human pituitary PRL. An initial peak or first surge of fascial PRL-like activity occurred between 4 and 15 d in primary culture. Ibuprofen, cytotoxic levels of 0.01% azide, or 7.5 mM EDTA and medium lacking serum [fetal bovine serum (FBS)] significantly (P less than or equal to 0.05) reduced PRL-like activity levels, whereas female steroids, 257 to 342 milliosmolarity, 1 to 3.6 mg/ml glucose, 2 to 20% FBS, and dialyzed FBS (MWCO approximately 1 kDa) were without effect. Optimum production of PRL-like activity occurred at pH 7.3. A second surge began after 18 d and continued until passage indicating that perhaps two populations of cells produced PRL-like activity in primary culture. Production of PRL-like activity by cells from early passages (1 and 2) became detectable at confluence, was serum-dependent, showed two patterns (tonic, rising to plateau), and averaged 3.2 fg.cell-1.3 d-1 feed interval. Cells from late passages showed morphologic damage...Continue Reading

References

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Citations

Sep 1, 1991·Medical Hypotheses·R Paus
Oct 21, 2000·Endocrine Reviews·A Slominski, J Wortsman
Jun 1, 1996·The Journal of Investigative Dermatology·R G Richards, S M Hartman

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