PMID: 11326864May 1, 2001Paper

Observation of surface structure of retinoblastoma cell line

Yan ke xue bao = Eye science
L YongpingZ Jianliang

Abstract

We study the adhesion molecules on the surface of SO-Rb50 retinoblastoma cell line. The distribution of proteoglycans in the retinoblastoma SO-Rb50 cell line was analyzed by histochem-electron microscopy, using Colloidal Iron in combination with a series of enzyme digestions. In addition, immunohistochemistry was performed using a panel of specific antibodies including neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP), S-100 protein, fibronectin, laminin, and collagen IV. Immunohistochemical stains showed the most marked cytoplasmic reactivity of SO-Rb50 cells with anti-NSE and anti-S100. The cells member and surface was postive with anti-NSE. No reactivity was noted with antibodies against laminin, GFAP, and collagen IV. After incubated with colloidal iron solution, three types of colloidal iron-positive stained material could be distinguished based on differences in shape, size, electron density: (1) electron dense particles, (2) the larger colloidal iron-positive filaments, (3) the smaller colloidal iron-positive filaments, which were observed present on the tumor cells surface and in the extracellular matrix. We think that the cell surface proteoglycans is the main component of adhesion molecules of reti...Continue Reading

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