Observation of the multiple halogenation of peptides in the electrospray ionization source

Journal of Mass Spectrometry : JMS
Yury KostyukevichEugene Nikolaev

Abstract

The chlorination of peptides and proteins is an important posttranslational modification, which is a physiological signature of an enzyme myeloperoxidase and can serve as a potential biomarker of some diseases (Parkinson's disease, Alzheimer's disease, etc.). The quantification of the chlorinated peptides has been very challenging in part due to their low levels and artifacts associated with sample preparation. One of the most convenient and promising methods to detect and investigate the chlorinated peptides in the biological samples is the electrospray ionization (ESI) mass spectrometry coupled to the fragmentation techniques (collision-induced dissociation and electron capture dissociation/electron transfer dissociation). We have shown that if the chlorine anions are present in the solution, then the peptide can undergo the chlorination during the ESI ionization. The effect was found to depend on the values of electric potentials of metal parts of the ESI interface. It was found that the grounding of ESI syringe results in the formation of an additional electric loop leading to the electrolytic production of Cl2 and as a consequence the hypochlorous acid inside the ESI needle. Hypochlorous acid reacts with amino groups of pe...Continue Reading

References

Jul 14, 1995·The Journal of Biological Chemistry·N M DomiganA J Kettle
Sep 6, 2000·Journal of Mass Spectrometry : JMS·J F MoraJ B Fenn
Nov 10, 2000·Journal of the American Society for Mass Spectrometry·J Zhu, R B Cole
Jul 11, 2001·Journal of the American Society for Mass Spectrometry·G J Van BerkelP D Schnier
Oct 16, 2001·Chemical Research in Toxicology·D I Pattison, M J Davies
Jul 15, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dong-Kug ChoiSerge Przedborski
Aug 19, 2007·Analytical Chemistry·Gary J Van Berkel, Vilmos Kertesz
Sep 15, 2009·Journal of the American Society for Mass Spectrometry·John R Lloyd, Sonja Hess
Dec 8, 2009·Journal of the American Society for Mass Spectrometry·Rui Wang, Renato Zenobi
Jul 20, 2010·Annual Review of Analytical Chemistry·Mélanie AbonnencHubert H Girault
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·O M PanasenkoA V Sokolov
Feb 7, 2014·Analytical Chemistry·Yury KostyukevichEugene Nikolaev
Dec 18, 2014·Nature Communications·Alexandra MüllerLars Ingo Ole Leichert

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