PMID: 6971848Apr 1, 1981Paper

Observations of radiation-induced chromosome fragment loss in live mammalian cells in culture, and its effect on colony-forming ability

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
S J GroteC A Shaw

Abstract

Our preceding paper (Crote, Joshi, Revell and Shaw 1981) described a method for the direct scrutiny of live cultured mammalian cells with a microscope, and reported that all diploid Syrian hamster cells (BHK 21 C13) of a sample given 1.4 Gy of 220 kV X-rays in Gl reached post-radiation mitosis without discernible abnormality, but then diverged in observed behaviour: descendent cells from some first mitoses continued to proliferate normally while cells from other first mitoses behaved abnormally and produced either slow-growth or stop-growth colonies. This paper completes our study of the same irradiated cell sample, and shows that these post-mitotic differences in clonogenic ability were related to acentric chromosome fragment losses at post-radiation mitosis, which were detected in live daughter-cell pairs as micronuclei. The proportion of live daughter-cell pairs scored as deficient was at least 80 per cent of the proportion of comparable fixed-and-stained mitoses with detected acentric fragments.

References

Sep 26, 1964·Nature·M STOKER, I MACPHERSON

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Citations

Mar 1, 1996·International Journal of Radiation Biology·M Jamali, K R Trott
Mar 1, 1996·International Journal of Radiation Biology·K SchwenkeL E Feinendegen
Feb 1, 1993·International Journal of Radiation Biology·I Spadinger, B Palcic
Oct 15, 1994·International Journal of Radiation Oncology, Biology, Physics·R WurmJ H Peacock
Feb 22, 2011·Comptes rendus biologies·Adeline GranzottoNicolas Foray
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D N Floyd, A M Cassoni
Sep 1, 1993·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·K SkladowskiG G Steel
Feb 13, 2016·International Journal of Radiation Oncology, Biology, Physics·UNKNOWN COPERNIC project investigatorsNicolas Foray
Jan 15, 2008·Radiation and Environmental Biophysics·Otilia Nuta, Firouz Darroudi
Mar 10, 1999·British Journal of Cancer·J L SchwartzR R Weichselbaum
Jan 1, 1985·Radiation and Environmental Biophysics·U Bertsche
Jan 1, 1994·Radiation and Environmental Biophysics·J Z Beer, I Szumiel
Feb 1, 1996·Physics in Medicine and Biology·G G Steel
Mar 26, 2011·Journal of Radiation Research·Mira MaaloufNicolas Foray
Apr 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S J GroteC A Shaw
Oct 1, 1990·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·S Powell, T J McMillan

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