Observations on the Early Establishment of Foliar Endophytic Fungi in Leaf Discs and Living Leaves of a Model Woody Angiosperm, Populus trichocarpa (Salicaceae)

Journal of Fungi
Yu-Ling HuangA Elizabeth Arnold

Abstract

Fungal endophytes are diverse and widespread symbionts that occur in the living tissues of all lineages of plants without causing evidence of disease. Culture-based and culture-free studies indicate that they often are abundant in the leaves of woody angiosperms, but only a few studies have visualized endophytic fungi in leaf tissues, and the process through which most endophytes colonize leaves has not been studied thoroughly. We inoculated leaf discs and the living leaves of a model woody angiosperm, Populus trichocarpa, which has endophytes that represent three distantly-related genera (Cladosporium, Penicillium, and Trichoderma). We used scanning electron microscopy and light microscopy to evaluate the timeline and processes by which they colonize leaf tissue. Under laboratory conditions with high humidity, conidia germinated on leaf discs to yield hyphae that grew epiphytically and incidentally entered stomata, but did not grow in a directed fashion toward stomatal openings. No cuticular penetration was observed. The endophytes readily colonized the interiors of leaf discs that were detached from living leaves, and could be visualized within discs with light microscopy. Although they were difficult to visualize within the ...Continue Reading

References

Jun 29, 2002·Science·Virginie S VervoortAnand K Srivastava
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Fabienne VailleauDominique Roby
Nov 26, 2002·Science·Regina S RedmanJoan M Henson
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·A Elizabeth ArnoldEdward Allen Herre
Mar 24, 2004·Nature Reviews. Microbiology·Gary E HarmanMatteo Lorito
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Frank WallerKarl-Heinz Kogel
Feb 25, 2009·The New Phytologist·R J RodriguezR S Redman
Jun 25, 2010·Current Opinion in Biotechnology·Weiqing ZengSheng Yang He
Oct 20, 2010·Molecular Plant-microbe Interactions : MPMI·Estrella LunaJurriaan Ton
Jul 21, 2012·Studies in Mycology·K BenschP W Crous
Oct 17, 2013·Nature Reviews. Microbiology·Martha C Giraldo, Barbara Valent
Mar 1, 1971·Planta·O L LangeL Kappen
Dec 17, 2014·Studies in Mycology·C M VisagieR A Samson
Nov 14, 2015·The New Phytologist·Posy E BusbyGeorge Newcombe

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Citations

May 23, 2021·Global Change Biology·Natalie ChristianKaty D Heath
Nov 29, 2021·The New Phytologist·Rosanne A HealyMatthew E Smith

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Methods Mentioned

BETA
light
scanning electron microscopy
light microscopy
PCR

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