PMID: 6162806Aug 1, 1980Paper

Observations on the EB virus envelope and virus-determined membrane antigen (MA) polypeptides

International Journal of Cancer. Journal International Du Cancer
J R NorthM A Epstein

Abstract

Experiments have been carried out to identify the polypeptide components of the Epstein-Barr (EB) virus-determined membrane antigen (MA) complex. Cells were radioiodinated using lactoperoxidase and the 125I-labelled surface antigens, released by Triton X100, were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) after complexing either with human sera having anti-MA activity or with a rabbit antiserum to EB virus. Using cells carrying EB virus isolated from four different conditions, including for the first time nasopharyngeal carcinoma (NPC), we have demonstrated four major polypeptides. One, with a molecular weight of 85,000, was remarkedly constant irrespective of the cell line from which it came; two, with molecular weights varying from 240,000 to 270,000 and from 320,000 to 340,000, showed minor differences in mobility apparently depending on the species of origin of the cells (human or marmoset), rather than disparity between strains of virus. A fourth component, of 160,000 daltons, was found on only two of the cell lines studied. In addition, it has been shown for the first time that the same polypeptides composing the MA complex are present on the viral envelope itself. The fact that the ...Continue Reading

References

Oct 1, 1978·Journal of Virology·G R Pearson, L F Qualtiere
Feb 15, 1975·International Journal of Cancer. Journal International Du Cancer·K Nilsson, J Pontén
Apr 1, 1976·Journal of the National Cancer Institute·M A Epstein
Sep 15, 1979·International Journal of Cancer. Journal International Du Cancer·D H CrawfordJ L Thompson
Oct 11, 1979·Nature·D A Thorley-Lawson
Nov 1, 1979·Journal of Virology·D A Thorley-Lawson, C M Edson
Sep 15, 1973·International Journal of Cancer. Journal International Du Cancer·M A EpsteinL V Meléndez
Mar 1, 1966·Journal of Bacteriology·G Henle, W Henle
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G MillerM Lipman
Nov 1, 1972·The Journal of General Virology·D J Moss, J H Pope
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G MillerM Lipman
Feb 1, 1974·European Journal of Immunology·J C Jensenius, A F Williams
Oct 1, 1968·The Australian Journal of Experimental Biology and Medical Science·J H Pope
Mar 1, 1968·Analytical Biochemistry·M S Reid, R L Bieleski
Feb 1, 1964·Lancet·J V PULVERTAFT

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Citations

Jan 1, 1982·Medical Microbiology and Immunology·B Georg-Fries, N Mueller-Lantzsch
Sep 1, 1982·Journal of Virological Methods·J R NorthM A Epstein
Aug 15, 1998·Journal of Virological Methods·A D Wilson, A J Morgan
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L F QualtiereG R Pearson
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J R NorthM A Epstein
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S F SlovinJ H Vaughan
Jan 1, 1987·Critical Reviews in Clinical Laboratory Sciences·W J RichtsmeierE M Mazur
Feb 27, 1981·Biochemical and Biophysical Research Communications·B C StrnadR H Neubauer
Sep 15, 1981·International Journal of Cancer. Journal International Du Cancer·N Mueller-LantzschD G Braun
May 15, 1981·International Journal of Cancer. Journal International Du Cancer·A B RickinsonM A Epstein
Mar 22, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M A Epstein
Jan 1, 1984·Annals of the New York Academy of Sciences·M A Epstein
Feb 12, 2015·Clinical & Translational Immunology·Jeffrey I Cohen
May 1, 1983·Journal of Virology·C M Edson, D A Thorley-Lawson

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