Observations on the presence of the peritrophic membrane in larval Trichoplusia ni and its role in limiting baculovirus infection

Journal of Invertebrate Pathology
P Wang, R R Granados

Abstract

Light microscopical examinations of dissected and stained peritrophic membranes (PMs) were conducted to determine the presence or absence of this protective structure in larvae of Trichoplusia ni, prior to and through ecdysis. Observations of fourth- and fifth-instar larvae of T. ni from two independent rearing colonies showed that PMs were present and lined the midgut prior to, during, and immediately after ecdysis in both instars. Western blot analysis of insect intestinal mucin (IIM), a major protective protein in the T. ni PM, indicated that synthesis of IIM occurred during T. ni embryonic development, or more precisely, that IIM synthesis started approximately 4 h prior to hatching. These results demonstrated that the neonate T. ni midgut is lined with a protective mucinous layer at hatching. A baculovirus enhancin from T. ni granulosis virus (TnGV) enhanced per os viral infections of budded viruses (BVs) of Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and T. ni single nuclear polyhedrosis virus (TnSNPV) in neonate, fourth-, and fifth-instar larvae of T. ni. These results provided further evidence that the PM may serve as a partial barrier to viruses in the midgut lumen and that enhancins can facilit...Continue Reading

References

Jan 1, 1991·Annual Review of Microbiology·H A Wood, R R Granados
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Nov 1, 1995·The Journal of General Virology·P W RoelvinkR R Granados
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Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·P Wang, R R Granados
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Dec 24, 1997·The Journal of General Virology·P WangR R Granados

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Citations

Apr 1, 2008·Journal of Insect Physiology·Ruth PlymaleKelli Hoover
Sep 20, 2001·The Journal of General Virology·T LuqueD Winstanley
Aug 19, 2015·PloS One·Wipasiri SoonthornchaiPikul Jiravanichpaisal
Sep 10, 2003·International Reviews of Immunology·Thomas E Clarke, Rollie J Clem
May 29, 2001·Archives of Insect Biochemistry and Physiology·P Wang, R R Granados
Sep 1, 2017·Archives of Virology·Ma de Los Ángeles Bivian-HernándezMaría Cristina Del Rincón-Castro
Feb 18, 2019·Journal of Economic Entomology·Sóstenez A Vessaro-SilvaCamila Maria Toigo de Oliveira
Nov 24, 1999·The Journal of General Virology·W F IJkelJ M Vlak
Jul 14, 2019·Journal of Insect Physiology·Kotaro Konno, Wataru Mitsuhashi
Aug 20, 2020·Insect Biochemistry and Molecular Biology·Shaohua Wang, Ping Wang
Apr 20, 2021·Annual Review of Virology·Enhao MaGong Cheng
Oct 1, 2021·Neotropical Entomology·Priscila StinguelHugo José Gonçalves Dos Santos Júnior

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