Observer accuracy in estimating proportions in images: implications for the semiquantitative assessment of staining reactions and a proposal for a new system

Journal of Clinical Pathology
S S Cross

Abstract

The results of immunohistochemical staining are often assessed by semiquantitative scoring. However, these scoring systems are usually non-standardised and there has been little evaluation of the accuracy and reliability of this subjective assessment. To assess the accuracy of observer estimation of proportions of objects in an image. Images were generated that contained known proportions of pink squares in grids of 50 x 50 and 100 x 100 squares. Observers were shown each image for five seconds in random order and either estimated the proportion of pink squares or selected the image (from a pair of images) that contained the greater proportion of pink squares. The observers were four consultant histopathologists, seven trainee histopathologists, and six control non-histopathologists. The raw estimations of proportions showed a close correlation with the real proportions, with correlation coefficients of 0.94 and 0.95 for consultant and trainee histopathologists on the 50 x 50 grids. However, the performance in the comparison task was much higher, with an almost perfect classification for grids of equal size even when the proportions only differed by 5%. Histopathologists can estimate proportions of objects in an image with a re...Continue Reading

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Citations

May 27, 2003·The American Journal of Surgical Pathology·Peter N FurnessRafail Rosenthal
Mar 10, 2010·Biomarker Insights·Leandro Luongo de MatosMaria Aparecida da Silva Pinhal
Jun 20, 2014·Indian Journal of Pathology & Microbiology·Ibrahim Al HaddabiShyam Ganguly
May 2, 2008·Computers in Biology and Medicine·Binghan LiuWeizhi Wang
Feb 28, 2003·Histopathology·M Deolekar, J A Morris
Mar 7, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·U ForsumC Spiegel
Jan 10, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kyriakos ZafirellisAglaia Zachaki
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Seshi R SompuramSteven A Bogen
Mar 3, 2004·Journal of Magnetic Resonance Imaging : JMRI·Marrita M MahonNandita M deSouza
Nov 13, 2008·Expert Review of Molecular Diagnostics·Laoighse MulraneWilliam M Gallagher
Feb 5, 2019·ILAR Journal·David K Meyerholz, Amanda P Beck
Mar 22, 2014·Biomedical Reports·Luis Fernando Méndez-LópezManuel López-Cabanillas-Lomelí
Jul 18, 2021·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Josien C A JenniskensHeike I Grabsch

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