Observing heme doming in myoglobin with femtosecond X-ray absorption spectroscopy

Structural Dynamics
Matteo LevantinoMarco Cammarata

Abstract

We report time-resolved X-ray absorption measurements after photolysis of carbonmonoxy myoglobin performed at the LCLS X-ray free electron laser with nearly 100 fs (FWHM) time resolution. Data at the Fe K-edge reveal that the photoinduced structural changes at the heme occur in two steps, with a faster (∼70 fs) relaxation preceding a slower (∼400 fs) one. We tentatively attribute the first relaxation to a structural rearrangement induced by photolysis involving essentially only the heme chromophore and the second relaxation to a residual Fe motion out of the heme plane that is coupled to the displacement of myoglobin F-helix.

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Citations

Oct 7, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mahsa SilataniMajed Chergui
Jan 31, 2017·Journal of the American Chemical Society·Nicholas A MillerRoseanne J Sension
Oct 24, 2017·Reports on Progress in Physics·E A SeddonW Wurth
Jul 4, 2018·Chemistry : a European Journal·Eric Collet, Marco Cammarata
Aug 24, 2019·Physical Chemistry Chemical Physics : PCCP·Alexander BritzRoberto Alonso-Mori
May 20, 2017·Bioscience Reports·Michael A Hough
Aug 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Camila BacellarMajed Chergui
Jan 30, 2018·Structural Dynamics·Rafael AbelaLuc Patthey
Oct 31, 2018·Nature Communications·Konstantin FalahatiMiquel Huix-Rotllant
Dec 15, 2020·Physical Chemistry Chemical Physics : PCCP·Chantal Daniel
Feb 11, 2021·Faraday Discussions·Camila BacellarMajed Chergui
Jun 9, 2020·The Journal of Physical Chemistry. B·Shinya TaharaYasuhisa Mizutani
Mar 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Megan L ShelbyLin X Chen
Dec 19, 2019·The Journal of Physical Chemistry. B·Nicholas A MillerRoseanne J Sension
Aug 20, 2020·Nature Communications·Dominik KinschelMajed Chergui
May 26, 2018·The Journal of Physical Chemistry. a·Nicholas A MillerRoseanne J Sension
Aug 28, 2021·Science·Gisela Brändén, Richard Neutze
Nov 12, 2020·The Journal of Physical Chemistry. B·Nicholas A MillerRoseanne J Sension
Jul 11, 2019·The Journal of Physical Chemistry. B·Lindsay B MichockiRoseanne J Sension

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Methods Mentioned

BETA
X-ray
optical spectroscopy

Software Mentioned

ixppy
XPP
XFEL

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