Obstructor A organizes matrix assembly at the apical cell surface to promote enzymatic cuticle maturation in Drosophila.

The Journal of Biological Chemistry
Yanina-Yasmin PeschMatthias Behr

Abstract

Assembly and maturation of the apical extracellular matrix (aECM) is crucial for protecting organisms, but underlying molecular mechanisms remain poorly understood. Epidermal cells secrete proteins and enzymes that assemble at the apical cell surface to provide epithelial integrity and stability during developmental growth and upon tissue damage. We analyzed molecular mechanisms of aECM assembly and identified the conserved chitin-binding protein Obst-A (Obstructor A) as an essential regulator. We show in Drosophila that Obst-A is required to coordinate protein and chitin matrix packaging at the apical cell surface during development. Secreted by epidermal cells, the Obst-A protein is specifically enriched in the apical assembly zone where matrix components are packaged into their highly ordered architecture. In obst-A null mutant larvae, the assembly zone is strongly diminished, resulting in severe disturbance of matrix scaffold organization and impaired aECM integrity. Furthermore, enzymes that support aECM stability are mislocalized. As a biological consequence, cuticle architecture, integrity, and function are disturbed in obst-A mutants, finally resulting in immediate lethality upon wounding. Our studies identify a new cor...Continue Reading

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Citations

Oct 8, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Arzu Öztürk-ÇolakSofia J Araújo
Mar 18, 2016·Annual Review of Entomology·Kun Yan ZhuSubbaratnam Muthukrishnan
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