Abstract
Recently, several attempts have been made to create a generation of transgenic chickens via chimeric intermediates produced by primordial germ cells (PGCs) transfer. This study aimed to compare the influences of different chicken PGCs isolated from circulating blood (bPGCs) or gonads (gPGCs), purification (ACK, Percoll or trypsin) and transfection methods (electroporation or lipofection) on the expression of transgenes in vitro and the migration of modified donor cells to the recipient gonads. The highest average frequency of pEGFP-N1 plasmid-transfected bPGCs (75.8%) was achieved with Percoll density gradient centrifugation and electroporation. After ammonium chloride-potassium (ACK) treatment and lipofection, in vitro transgene expression was only detected in 35.2% of bPGCs. Chimeric chickens were produced from these purified, transfected and cultured cells, and the transgene was detected in the gonads of 44 and 42% of the recipient embryos that had been injected with bPGCs and gPGCs, respectively. These data confirmed that the combination of PGC purification via Percoll centrifugation and electroporation was an effective method for producing transgenic chickens. Subsequently, we used this method with expression vectors for g...Continue Reading
References
Apr 1, 1976·Developmental Biology·H Eyal-Giladi, S Kochav
Apr 1, 1990·Differentiation; Research in Biological Diversity·F ZaccantiA Quaglia
Dec 11, 1981·Science·J W Gordon, F H Ruddle
Nov 1, 1981·Cell·R L BrinsterR D Palmiter
Dec 1, 1995·Molecular Reproduction and Development·H KagamiR J Etches
Dec 22, 1998·Transgenic Research·Y H HongJ Y Han
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paul E Mozdziak, James N Petitte
Jun 12, 2004·EMBO Reports·Michael J McGrewHelen Sang
Feb 16, 2005·Theriogenology·Jin Nam KimJae Yong Han
Apr 23, 2005·Poultry Science·P E MozdziakJ N Petitte
Jun 9, 2006·Nature·Marie-Cecile van de LavoirRobert J Etches
Nov 30, 2006·Cell·Geraldine Seydoux, Robert E Braun
Apr 20, 2007·Biology of Reproduction·Yasuhiro YamamotoHiroshi Kagami
Mar 17, 2010·Journal of Bioscience and Bioengineering·Makoto MotonoShinji Iijima
Sep 21, 2010·BMC Biotechnology·Hee W SeoJae Y Han
Oct 5, 2010·PloS One·Jin Won ChoiJae Yong Han
Dec 3, 2010·PloS One·Joni MacdonaldMichael J McGrew
Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Isao Oishi
Jul 1, 2011·Biology of Reproduction·Jin Gyoung JungJae Yong Han
Jul 26, 2011·Animal Reproduction Science·C MatásJ Gadea
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joni MacdonaldMichael J McGrew
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tae Sub Park, Jae Yong Han
Jun 20, 2012·Polish Journal of Veterinary Sciences·L Chojnacka-PuchtaM Bednarczyk
Jun 29, 2013·Transgenic Research·Scott G TyackTimothy J Doran
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON
Citations
Apr 1, 2018·Reproduction in Domestic Animals = Zuchthygiene·E ŁukaszewiczM Bednarczyk
Jan 27, 2018·Journal of Applied Genetics·Marek BednarczykTakashi Kuwana
Jan 15, 2019·Journal of the Science of Food and Agriculture·Muhammad SaeedSun Chao
Mar 12, 2019·Journal of Cellular Biochemistry·Katarzyna StadnickaAnna Bajek
Sep 19, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Andrea SvoradováPeter Chrenek
Jan 12, 2020·Journal of Applied Genetics·Luiza Chojnacka-Puchta, Dorota Sawicka
Jun 1, 2021·Poultry Science·Marek BednarczykEwa Grochowska
Aug 7, 2021·Genes·Aleksandra DunislawskaMarek Bednarczyk