Occludin Content Modulates Hydrogen Peroxide-Induced Increase in Renal Epithelial Paracellular Permeability

Journal of Cellular Biochemistry
Danielle JanosevicKurt Amsler

Abstract

The ability of hydrogen peroxide (H2O2) to increase paracellular permeability of renal epithelial cell monolayers was examined and the role of occludin in this regulation was investigated. H2O2 treatment increased the paracellular movement of calcein, a marker for the leak pathway permeability, across monolayers of two renal epithelial cell lines, MDCK and LLC-PK1, in a concentration-dependent manner. At the same concentrations, H2O2 did not alter transepithelial resistance (TER) nor increase cell death. The magnitude of the H2O2-induced increase in leak pathway permeability was inversely related to cellular occludin protein content. H2O2 treatment did not produce any major change in total cellular content or Triton X-100-soluble or -insoluble fraction content of occludin protein. Occludin protein staining at the tight junction region was diminished following H2O2 treatment. The most dramatic effect of H2O2 was on the dynamic mobility of GFP-occludin into the tight junction region. H2O2 treatment slowed lateral movement of GFP-occludin into the tight junction region but not on the apical membrane. Further, removal of the cytoplasmic C-terminal region of occludin protein eliminated the effect of H2O2 on GFP-occludin lateral move...Continue Reading

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Citations

Jun 7, 2018·American Journal of Physiology. Cell Physiology·Sahar BilalKurt Amsler
Nov 23, 2019·Journal of Cell Science·Saranyaraajan VaradarajanAnn L Miller
Jul 16, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yin ShiHuangan Wu
Jul 25, 2021·International Journal of Molecular Sciences·Ashley MonacoKurt Amsler

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