PMID: 9548718May 23, 1998Paper

Occludin-deficient embryonic stem cells can differentiate into polarized epithelial cells bearing tight junctions

The Journal of Cell Biology
M SaitouS Tsukita

Abstract

Occludin is the only known integral membrane protein of tight junctions (TJs), and is now believed to be directly involved in the barrier and fence functions of TJs. Occludin-deficient embryonic stem (ES) cells were generated by targeted disruption of both alleles of the occludin gene. When these cells were subjected to suspension culture, they aggregated to form simple, and then cystic embryoid bodies (EBs) with the same time course as EB formation from wild-type ES cells. Immunofluorescence microscopy and ultrathin section electron microscopy revealed that polarized epithelial (visceral endoderm-like) cells were differentiated to delineate EBs not only from wild-type but also from occludin-deficient ES cells. Freeze fracture analyses indicated no significant differences in number or morphology of TJ strands between wild-type and occludin-deficient epithelial cells. Furthermore, zonula occludens (ZO)-1, a TJ-associated peripheral membrane protein, was still exclusively concentrated at TJ in occludin-deficient epithelial cells. In good agreement with these morphological observations, TJ in occludin-deficient epithelial cells functioned as a primary barrier to the diffusion of a low molecular mass tracer through the paracellular...Continue Reading

References

Mar 1, 1992·Journal of Virology·K Tsukiyama-KoharaA Nomoto
May 1, 1992·Molecular and Cellular Biology·R M MortensenJ G Seidman
Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B GumbinerD Apatira
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T YagiS Aizawa
Aug 18, 1989·Science·E Rodriguez-Boulan, W J Nelson
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Sauer, N Henderson
Oct 1, 1988·Molecular and Cellular Biochemistry·B R StevensonS Bullivant
May 19, 1988·Nature·S CitiJ Kendrick-Jones
Dec 1, 1987·The American Journal of Physiology·B Gumbiner
Jan 1, 1974·International Review of Cytology·L A Staehelin
Aug 25, 1981·Journal of Molecular Biology·N Sternberg, D Hamilton
Jan 27, 1984·Biochimica Et Biophysica Acta·A J Verkleij
Mar 1, 1982·Cell·P Pinto da Silva, B Kachar
Apr 1, 1982·Nature·B Kachar, T S Reese
Jul 1, 1980·The Histochemical Journal·K T Tokuyasu
Mar 24, 1995·Science·A G ReaumeJ Rossant
Dec 1, 1993·The Journal of Cell Biology·B M Gumbiner
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jan 1, 1993·Molecular and Cellular Biology·G KellerM V Wiles
Mar 1, 1993·Genes & Development·A WatakabeY Shimura
Oct 1, 1995·Current Opinion in Cell Biology·S K Kim
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J X Jiang, D A Goodenough
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Aug 1, 1996·The Journal of Cell Biology·B H KeonW W Franke
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E NellesK Willecke
Feb 6, 1997·Nature·A M SimonD L Paul
Jun 16, 1997·The Journal of Cell Biology·A SakakibaraS Tsukita

❮ Previous
Next ❯

Citations

May 20, 1999·The Anatomical Record·K S O'Shea
May 8, 2000·Journal of Cellular Biochemistry·M S BaldaK Matter
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawaguchiK Tanikawa
Oct 18, 2002·Journal of Cellular Physiology·Nicole S HarhajDavid A Antonetti
Sep 28, 2007·Histochemistry and Cell Biology·Kiyoko Uehara, Akira Uehara
Apr 17, 2008·Histochemistry and Cell Biology·Carola Förster
Sep 25, 2010·Journal of Neural Transmission·H C BauerH Bauer
Apr 25, 2009·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Satoshi OhtaniMitsukazu Gotoh
Mar 7, 2007·Angiogenesis·Kathryn K EricksonDavid A Antonetti
Apr 20, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Silvia S Kang, Dorian B McGavern
Apr 4, 2008·Pathology Oncology Research : POR·Erika OrbánAndrás Kiss
Apr 12, 2005·Advanced Drug Delivery Reviews·Gemma J FeldmanMichael P Ryan
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Nicole S Harhaj, David A Antonetti
Nov 18, 2005·Brain Research. Brain Research Reviews·Char-Huei LaiJoyce M Leo
May 14, 2003·Experimental Eye Research·Yuriko BanShigeru Kinoshita
Jun 16, 2000·Advanced Drug Delivery Reviews·L A Lapierre
Jun 16, 2000·Advanced Drug Delivery Reviews·H ClarkeJ M Mullin
Dec 31, 2003·Hearing Research·Shin-iciro KitajiriShoichiro Tsukita
Oct 6, 1999·Current Opinion in Cell Biology·S TsukitaM Itoh
Nov 21, 2000·Current Opinion in Neurobiology·J M Bronstein
Jan 20, 2000·Trends in Cardiovascular Medicine·G BazzoniE Dejana
Oct 26, 2000·Pharmaceutical Science & Technology Today·P D WardD R Thakker
Feb 27, 2004·Journal of Mammary Gland Biology and Neoplasia·Masahiko Itoh, Mina J Bissell
Mar 1, 2012·Cell Death & Disease·N BeemanH K Baumgartner
Oct 27, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vassiliki N TzelepiChrisoula D Scopa
Jul 19, 2002·Nature·Mitinori SaitouM Azim Surani
Apr 12, 2003·Anatomical Science International·Yoshiro ToyamaShigeki Yuasa
Jun 9, 2000·Kidney International·L Gonzalez-MariscalJ L Reyes
Apr 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen L EdelblumJerrold R Turner
Jun 28, 2002·Acta histochemica·Megan D Orchard, Christopher R Murphy
Mar 24, 2009·Journal of Neurovirology·Nathan S IveyAndrew A Lackner
Feb 7, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cécile BeaurepaireDerek M McKay
Jun 12, 2002·Molecular Biology of the Cell·Anne-Marie MarzescoAhmed Zahraoui

❮ Previous
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