Occult endotoxin in bacterial protoplasm.

Journal of Bacteriology
J RudbachE RIBI

Abstract

Protoplasm separated from disrupted cells of gram-negative bacteria was extracted with hot phenol-water or was precipitated with ethyl alcohol after digestion with Pronase. These methods recovered about 10 times more endotoxin than was detectable in the untreated protoplasm. Inactivation of endotoxin by protoplasm also occurred in vitro when the endotoxin was first dissociated into subunits before reaction with protoplasm. Despite this increased yield from another source, the major proportion of endotoxin was still found in the cell walls.

References

Jun 30, 1966·Annals of the New York Academy of Sciences·J A RudbachE Ribi
Oct 1, 1966·Journal of Bacteriology·J A Rudbach, A G Johnson
Dec 1, 1966·The Journal of Infectious Diseases·K C Milner, R A Finkelstein
May 23, 1964·Nature·J A RUDBACH, A G JOHNSON
Dec 1, 1964·Journal of Bacteriology·R L ANACKERP W STASHAK

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