Occult hepatitis B virus infection: influence of S protein variants

Virology Journal
Zhenhua ZhangXu Li

Abstract

In occult hepatitis B viral infection (OBI), the persistence of hepatitis B virus (HBV) DNA is associated with a lack of hepatitis B surface antigen (HBsAg). To assess the possible role of HBsAg immune escape variants in OBI patients, variability in the HBV S gene was evaluated for OBI patients as well as chronic HBV infection patients from the same families. We selected 17 HBV DNA-positive/HBsAg-negative patients (OBI group) and 15 HBV DNA- and HBsAg-positive patients from OBI families (control group). The S gene was amplified and cloned, and at least 15 clones per patient were sequenced and analyzed. Although the incidence of stop codon mutations within the S region was higher in the OBI group (13.6 %) than in the control group (1.5 %, P < 0.001), this type of mutation, together with insertion and deletion mutations, was prevalent in only three OBI patients. In the major hydrophilic region (MHR), a median of 0.75 residues were altered in every 100 residues for the OBI patients, whereas 0.95 out of 100 residues were changed in the control group (P = 0.428). Furthermore, some variants that are generally considered immune escape variants, such as mutations at positions s145, s147, and s123, were only observed in less than 5 % of...Continue Reading

References

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Citations

Oct 16, 2016·Archives of Virology·Zhenhua ZhangJun Li
Feb 16, 2018·Journal of Medical Virology·Carla de Castro Sant' AnnaLuisa Caricio Martins
Oct 4, 2017·Journal of Medical Virology·Edina Amponsah-DacostaMaphahlaganye J Mphahlele
Jan 29, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·An-Qun HuYing-Jie Zheng
Jun 3, 2021·Diagnostics·Maria V KonoplevaAnatoly P Suslov

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Datasets Mentioned

BETA
GQ205441

Methods Mentioned

BETA
ELISA
PCR
PCRs
enzyme-linked immunosorbant assay
electrophoresis

Software Mentioned

SPSS
Tanon
Primer5
Image

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