Occurrence and abundance of ammonia-oxidizing archaea and bacteria from the surface to below the water table, in deep soil, and their contributions to nitrification

MicrobiologyOpen
Lei ZhengTao Xu

Abstract

Using molecular biology methods (qualitative and quantitative PCR), we determined the occurrence and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) from a dry inland soil in Basel, Switzerland, and from the riparian zone of Baiyangdian Lake, China. We also determined the contributions of these microorganisms to ammonia oxidization at different depths based on the nitrification rate. The number of archaeal amoA genes (the key functional gene in AOA) was larger than the number of bacterial amoA genes in each sample, suggesting a dominant role for the AOA amoA gene in environments with a low ammonium concentration. In Baiyangdian Lake, the number of archaeal amoA genes was highest at 6 m and lowest at 12 m from the land-water interface in the soil (at depths from 40 to 60 cm), close to the groundwater, which suggests that AOA become more competitive in environments with a low dissolved oxygen content and are promoted by low pH. The nitrification rate was significantly negatively correlated with depth in the Baiyangdian Lake soil and significantly positively correlated with the number of AOB amoA genes at this site, 6 m from the water.

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Citations

Mar 6, 2019·Archaea : an International Microbiological Journal·Aline BelmokCynthia M Kyaw
Aug 11, 2020·World Journal of Microbiology & Biotechnology·Elizabeth Temitope AloriOlubukola Oluranti Babalola
Jul 28, 2021·Environmental Geochemistry and Health·Guigui XuHaiting Liang

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Methods Mentioned

BETA
electrophoresis
Illumina sequencing
PCR

Software Mentioned

DOTUR
SPSS
MEGA
SigmaPlot
Clustal
BLAST

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