Occurrence and characterisation of ESBL-encoding plasmids among Escherichia coli isolates from fresh vegetables

Veterinary Microbiology
Christin FreitagStefan Schwarz

Abstract

Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates have been increasingly reported in different reservoirs. The aims of this study were to investigate the presence of ESBL-producing E. coli in fresh vegetables and to characterise their ESBL gene-carrying plasmids. Among the 245 samples from vegetables investigated during 2011-2013, seven putative ESBL-producing E. coli (salad n = 2, sprouts n = 5) were found. They were subjected to ESBL phenotypic confirmatory tests, detection/sequencing of ESBL genes, antimicrobial susceptibility testing (AST), phylotyping, XbaI-macrorestriction analysis, multilocus sequence typing and transformation. Transformants were characterised by AST, S1-nuclease PFGE, replicon typing, conjugation and investigated for co-located antimicrobial resistance genes. Two ESBL gene-carrying plasmids were sequenced using a HiSeq 2500 system. The seven isolates were confirmed as ESBL producers, displayed unrelated XbaI-patterns and unique sequence types (STs) and belonged to the phylogroups A, B1 or D. The ESBL genes were located on plasmids. Two plasmids carrying blaCTX-M-14 genes (incompatibility group IncK or IncHI2) were seen in isolates from salad (ST973) and sprout (ST527). Two blaCTX-...Continue Reading

Citations

Sep 26, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ashley C CormierPatrick Boerlin
Feb 18, 2020·Frontiers in Microbiology·Cameron J ReidSteven P Djordjevic
Nov 1, 2020·MicrobiologyOpen·Katrin ZurfluhRoger Stephan
Jul 30, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Daniel MartakUNKNOWN MODERN WP3 study group

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