Occurrence and characteristics of extended-spectrum β-lactamase- and carbapenemase- producing bacteria from hospital effluents in Singapore

The Science of the Total Environment
Laurence HallerKarina Yew-Hoong Gin

Abstract

One of the most important resistance mechanisms in Gram-negative bacteria today is the production of enzymes causing resistance to cephalosporin and carbapenem antibiotics. The spread of extended-spectrum β-lactamases (ESBL)- and carbapenemase- producing Gram-negative bacteria is an emerging global public health problem. The aim of the present study was to (i) assess the prevalence of carbapenem-resistant bacteria (CRB) and ESBL-producing strains in sewage effluents from two major hospitals in Singapore, (ii) characterize the isolated strains and (iii) identify some of the ESBL and carbapenemase genes responsible for the resistance. CHROMagar ESBL and KPC plates were used to rapidly screen for ESBL-producing bacteria and those expressing reduced susceptibility to carbapenems, respectively. The abundance of ESBL-producers and CRB in hospital wastewater ranged between 103 and 106CFU/mL. Out of the 66 isolates picked from ESBL and KPC plates, 95%, 82%, 82% and 76% were resistant to ceftriaxone, ceftazidime (3rd generation cephalosporin family), ertapenem and meropenem (carbapenem family), respectively. Among the resistant isolates, the most predominant taxa identified were Pseudomonas spp. (28.2%), Klebsiella spp. (28.2%), Enterob...Continue Reading

Citations

May 18, 2020·Environmental Monitoring and Assessment·Guilherme Sgobbi ZaguiSusana Inés Segura-Muñoz
Dec 12, 2018·Microbiology Resource Announcements·Charmaine NgKarina Yew-Hoong Gin
Oct 21, 2020·Antibiotics·Florence Hammer-DedetPatricia Licznar-Fajardo
Oct 30, 2020·International Journal of Environmental Research and Public Health·Kristína LépesováLucia Bírošová
Oct 27, 2020·Current Opinion in Environmental Science & Health·Paola Verlicchi
Mar 2, 2021·The Science of the Total Environment·Takashi Azuma, Tetsuya Hayashi
Mar 20, 2021·Travel Medicine and Infectious Disease·Samantha Dos Santos Tufic-GaruttiKáris Maria de Pinho Rodrigues
Apr 26, 2021·Journal of Global Antimicrobial Resistance·Zineb CherakJean-Marc Rolain
Jun 16, 2021·Archives of Microbiology·Zimeng HuWei Zhang
Aug 27, 2021·Journal of Global Antimicrobial Resistance·Zineb CherakJean-Marc Rolain

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