Abstract
The investigation of the causative agents for foodborne diseases and subsequent development of preventive steps to control the outbreak and related economic loss is the basic goal and priority of a rational food safety program. The entero-pathogenic Vibrio spp., which are Gram negative bacteria inhabiting estuarine ecosystems, are the major cause of foodborne illness associated with the consumption of raw or undercooked contaminated seafood or shellfish. To survey the Vibrio contamination in sea snails (Neptunea cumingi Crosse and Busycon canaliculatu), a total of 20 samples were collected from traditional market, at Qingdao city in Shandong province, China and analyzed for Vibrio species contamination. Presumptive-positive colonies grown on a specific Vibrio agar-based medium were picked and identified by the VITEKTM. Vibrio alginolyticus, V. parahaemolyticus, and V. vulgaris were isolated and identified in 11, 8, and 2 seafood samples, respectively. Among the 8 isolates of V. parahaemolyticus. The V. parahaemolyticus isolates were further tested for the tdh, trh, and tlh virulence factors. All the V. parahaemolyticus isolates were tlh-positive, however, all of them were tdh-negative. Interestingly 2 V. parahaemolyticus isolat...Continue Reading
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