Occurrence and molecular characterization of free-living amoeba species (Acanthamoeba, Hartmannella, and Saccamoeba limax) in various surface water resources of Iran

Parasitology Research
Mohammad Reza MahmoudiPanagiotis Karanis

Abstract

This study was conducted to determine the presence and molecular identity of Acanthamoeba species in the surface water resources of four provinces in Iran, namely Guilan, Mazandaran (North of Iran), Alborz, and Tehran (capital city), using culture- and molecular-based methods. During March to November 2014, 49 surface water samples were collected from environmental water sources-the distinct surface waters of Guilan, Mazandaran, Alborz, and Tehran provinces, in Iran. For the isolation of Acanthamoeba species, approximately 500 ml of the water samples were filtered through a cellulose nitrate membrane with a pore size of 0.45 μ. The filter was transferred onto non-nutrient agar plates seeded with Gram-negative bacteria (Escherichia coli) as a food source. The presence of Acanthamoeba was confirmed by the genus-specific primer pair JDP1 and 2, and/or NA primers were used to identify Acanthamoeba and certain other free-living amoebae. In total, 38 out of 49 samples were positive by culture and/or PCR for Acanthamoeba and other free-living amoebae from all three provinces. By sequencing the positive isolates, the strains were shown to belong to Acanthamoeba (16 isolates belonged to T4 and 2 isolates belonged to T5), Hartmannella ve...Continue Reading

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Citations

Jan 19, 2016·Parasitology Research·Thani ThammaratanaUNKNOWN Neglected, zoonosis and vector-borne disease research group
Jun 10, 2016·Water Research·Judit Plutzer, Panagiotis Karanis
Mar 5, 2016·Experimental Parasitology·Monika DerdaElżbieta Wandurska-Nowak
Nov 9, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Ehsan SaburiHossein Vazini
Oct 5, 2018·Journal of Water and Health·Samira DodangehSeyed Abdollah Hosseini
Oct 17, 2017·Acta Parasitologica·Ali HanilooElnaz Kadkhodamohammadi
Apr 18, 2020·Journal of Water and Health·İlknur KoyunPanagiotis Karanis
Oct 23, 2019·Journal of Water and Health·Hadi MirahmadiHarlan R Barker
Jun 23, 2021·Acta Parasitologica·Marianna de Carvalho ClímacoSilvio Santana Dolabella

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