Occurrence, Identification, and Antimicrobial Resistance Profiles of Extended-Spectrum and AmpC β-Lactamase-Producing Enterobacteriaceae from Fresh Vegetables Retailed in Gauteng Province, South Africa

Foodborne Pathogens and Disease
Loandi RichterLise Korsten

Abstract

Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase-producing Enterobacteriaceae are no longer restricted to the health care system, but represent increased risks related to environmental integrity and food safety. Fresh produce has been increasingly reported to constitute a reservoir of multidrug-resistant (MDR) potential human pathogenic Enterobacteriaceae. This study aimed to detect, identify, and characterize the antimicrobial resistance of ESBL/AmpC-producing Enterobacteriaceae isolates from fresh vegetables at point of sale. Vegetable samples (spinach, tomatoes, lettuce, cucumber, and green beans; n = 545) were purchased from retailers in Gauteng, the most densely populated province in South Africa. These included street vendors, trolley vendors, farmers' market stalls, and supermarket chain stores. Selective enrichment, plating onto chromogenic media, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) confirmation of isolate identities showed that 17.4% (95/545) vegetable samples analyzed were contaminated with presumptive ESBL/AmpC-producing Enterobacteriaceae. Dominant species identified included Escherichia coli, Enterobacter cloacae, Enterobacter asburiae, and Klebsi...Continue Reading

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Citations

Oct 29, 2020·Foodborne Pathogens and Disease·Ningbo LiaoLee W Riley
Apr 26, 2020·The Science of the Total Environment·Houpu ZhangHua Fang
May 1, 2021·International Journal of Environmental Research and Public Health·Kashaf JunaidAbdul Rehman
Aug 9, 2021·Journal of Applied Microbiology·Marcelo Belchior Rosendo da SilvaBernadette Dora Gombossy de Melo Franco

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

BLAST
Chromas
BioEdit

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