Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa.

Journal of Clinical Microbiology
M Y LamJ S Lam

Abstract

The lipopolysaccharide (LPS) of Pseudomonas aeruginosa PAO1 contains two species of O polysaccharide termed A and B bands. The high-molecular-weight B-band LPS determines the O specificity of the bacterium, while the antigenically distinct A-band LPS consists of only shorter-chain polysaccharides. Seven hybridomas secreting A-band-specific monoclonal antibodies were produced and used to study the LPS of standard and clinical strains. Although A-band antibodies did not agglutinate any of the serotype strains presently in the International Antigenic Typing Scheme, Western immunoblots revealed that 11 of the 17 serotype strains possessed A-band LPS. In a group of 250 clinical isolates from patients with cystic fibrosis, 170 (68%) had A-band LPS on the basis of agglutination tests, but in silver-stained gels all were shown to be deficient in O-antigen-containing B band. Investigation of serial isolates from a single patient revealed a pattern of antigenic variation. During the course of the infection, serotypeable isolates became nontypeable, and the O antigen was replaced with A band as the major LPS antigen. These results suggest that A-band LPS may be the major LPS antigen in nontypeable clinical isolates and a common antigen am...Continue Reading

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Citations

Jan 1, 1993·The Journal of Applied Bacteriology·I R Poxton
Feb 6, 1998·FEMS Microbiology Reviews·E S Stanislavsky, J S Lam
Jul 1, 1997·FEMS Microbiology Reviews·T J BeveridgeZ Li
Aug 28, 2009·Innate Immunity·Jerry D KingJoseph S Lam
Feb 6, 2020·Antimicrobial Agents and Chemotherapy·Gabrielle RichardGreg Hussack
Feb 4, 2003·Journal of Bacteriology·David H SpencerMaynard V Olson
Jan 22, 2003·Environmental Microbiology·Craig DanielsJoseph S Lam
Sep 27, 2002·Applied and Environmental Microbiology·R Sean NormanPamela J Morris
Nov 2, 2014·Canadian Journal of Microbiology·Salim T Islam, Joseph S Lam
Jun 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A BraunerB Wretlind
Oct 31, 2002·Molecular Microbiology·Antonio DiGiandomenicoPeter Castric
Apr 21, 2007·Journal of Medical Microbiology·Abbie M WeisnerTyrone L Pitt
Dec 11, 2007·The Journal of Biological Chemistry·Wayne L MillerJoseph S Lam
Sep 16, 2015·Antimicrobial Agents and Chemotherapy·Keith PooleJoseph S Lam

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