PMID: 9435105Jan 22, 1998Paper

Occurrence of an antigenic triacyl trehalose in clinical isolates and reference strains of Mycobacterium tuberculosis

FEMS Microbiology Letters
M MuñozMamadou Daffé

Abstract

A careful re-examination of the glycolipid content of clinical isolates and reference strains of the tubercle bacillus, Mycobacterium tuberculosis, led to the identification of a glycoconjugate that passed unnoticed in earlier studies. Nuclear magnetic resonance spectroscopy, gas chromatography-mass spectrometry and chemical degradations were used to identify the glycolipid as a 2,3,6-triacyl trehalose. The glycolipid contains a phthienoic acyl substituent, a family of multimethyl-branched, alpha,beta-unsaturated fatty acids specific for virulent strains of the tubercle bacillus. It reacted with sera from tuberculosis patients with a specificity and sensitivity of 96.2% and 76%, respectively. Comparable data were obtained with the 2,3-diacyl trehaloses of M. tuberculosis and M. fortuitum and with the triacyl trehaloses of M. fortuitum, suggesting that the antigens from the latter species may be used for the serodiagnosis of tuberculosis.

References

Aug 21, 1992·Science·B R Bloom, C J Murray
Apr 1, 1992·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M RidellM Magnusson
Oct 1, 1989·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·P CruaudH L David
Mar 15, 1988·European Journal of Biochemistry·M DafféG Lanéelle
Oct 1, 1996·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M LuquinV Ausina
Dec 1, 1996·American Journal of Respiratory and Critical Care Medicine·L EscamillaL M López-Marín
Nov 5, 1997·Advances in Microbial Physiology·M Daffé, P Draper

❮ Previous
Next ❯

Citations

Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·R SaavedraL M López-Marín
Jun 11, 2014·Critical Reviews in Biochemistry and Molecular Biology·Shiva Kumar AngalaMary Jackson
Jul 17, 2015·BioMed Research International·Milena Maya-HoyosCarlos Y Soto
May 29, 2002·Chemistry & Biology·David E MinnikinGurdyal S Besra
May 18, 2018·Future Microbiology·Parul SinghSangita Mukhopadhyay
Mar 3, 2020·Organic & Biomolecular Chemistry·Santanu Jana, Suvarn S Kulkarni
Jul 1, 2021·Chemical Reviews·Mira HolzheimerAdriaan J Minnaard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.