Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil

Veterinary Parasitology
S M GennariEliana M C Villalobos

Abstract

The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.

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Citations

Dec 12, 2018·Parasite : Journal De La Société Française De Parasitologie·Qing-Feng MengXiao-Feng Shan
Dec 29, 2016·Acta Parasitologica·Jonatas C AlmeidaRafael F C Vieira
Sep 17, 2020·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Igor Falco ArrudaPatricia Riddell Millar
Jun 24, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Manoel Junqueira Maciel RibeiroAntônio Marcos Guimarães
Oct 23, 2020·Animals : an Open Access Journal From MDPI·Sharon Tirosh-LevyMonica Leszkowicz Mazuz
Sep 30, 2018·Acta Tropica·Thiago Rocha MoreiraAntonio Humberto Hamad Minervino
Feb 26, 2020·Food and Waterborne Parasitology·S StelzerG Schares
Feb 23, 2020·Experimental Parasitology·Mariana BernsteinGastón Moré
Aug 18, 2020·Research in Veterinary Science·J P DubeyO C H Kwok

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