Occurrence of Arabis mosaic virus and Grapevine leaf roll associated virus-3 on Grapevines in Iran

Plant Disease
R PourrahimA R Golnaraghi

Abstract

During 2001, a survey was conducted in vineyards in northwestern Iran and the eastern and western provinces of Azarbaijan, Zanjan, and Qazvin to detect the presence of Arabis mosaic virus (ArMV) and Grapevine leaf roll associated virus-3 (GLRaV-3). From December 2001 through March 2002, 5,352 dormant stem cuttings were collected. A portion of all stem cuttings was callused, rooted, potted, and grown in a greenhouse. Each sample was tested for the presence of ArMV and GLRaV-3 with specific antisera (Bioreba, Basel, Switzerland). Extracts of bark scrapings were prepared from the remaining portion of the dormant cuttings. After bud break of rooted cuttings, leaf extracts were prepared by the method used by Rowhani et al. (2). Dormant bark and leaf extracts were used with double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Among the samples tested, ArMV and GLRaV-3 were found in 4.7 and 2.3% of the collection, respectively. Leaf extracts that had tested positive for ArMV using ELISA, were mechanically inoculated on the indicator host plants of Chenopodium amaranticolor, Cucumis sativus, and Petunia × hybrida. All plants developed local lesions that subsequently developed systemic chlorosis that is reported for A...Continue Reading

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