Abstract
Bacteriophage-based microbial source-tracking studies are an economical and simple way of identifying fecal sources in polluted water systems. Recently isolated Bacteroides spp. strains ARABA 84, and GB-124 have been shown to detect bacteriophages exclusively in aquatic systems impacted by human fecal material. To date, limited examination of the occurrence or concentration of phages capable of infecting Bacteroides fragilis strain GB-124 or B. thetaiotaomicron strain ARABA 84 in human and animal feces has been carried out. This study reports the prevalence rates and concentrations of phages infecting ARABA 84 and GB-124 host strains in human and a range of animal feces. Discrete human fecal samples (n=55) and pooled animal samples (n=46, representing the feces of over 230 animals) were examined for phages infecting the host strains ARABA 84, GB-124, and E. coli strain WG5. Both human Bacteroides host strains were highly specific (95% and 100% for ARABA 84 and GB-124, respectively), challenging results from previous studies. This study supports the use of Bacteroides strains GB-124 and ARABA 84 in fecal source tracking studies for the detection of human fecal contamination.
References
Jul 1, 1987·Applied and Environmental Microbiology·C Tartera, J Jofre
Mar 1, 1986·The Journal of Applied Bacteriology·A H HavelaarW M Hogeboom
Sep 1, 1983·The Journal of General Virology·K FuruseI Watanabe
Apr 2, 1999·Applied and Environmental Microbiology·A PuigR Araujo
Jan 3, 2001·Applied and Environmental Microbiology·H StenderJ Coull
Jun 19, 2002·International Journal of Hygiene and Environmental Health·Christophe GantzerLouis Schwartzbrod
Nov 8, 2002·Water Research·John E WhitlockValerie J Harwood
Nov 27, 2002·Applied and Environmental Microbiology·Troy M ScottJerzy Lukasik
Sep 10, 2005·Applied and Environmental Microbiology·Andrey PayanMaite Muniesa
Jun 6, 2006·Applied and Environmental Microbiology·Alice LaytonGary Sayler
Dec 7, 2006·Epidemiology·John M ColfordStephen B Weisberg
Feb 6, 2007·Water Research·James EbdonHuw Taylor
Jul 24, 2007·Water Research·Katharine G Field, Mansour Samadpour
Jan 23, 2008·Journal of Water and Health·Samir H Moussa, Rene D Massengale
Jan 29, 2010·Water Science and Technology : a Journal of the International Association on Water Pollution Research·N BonillaG A Toranzos
May 11, 2010·Water Research·Kannappan VijayavelHuw Taylor
Jan 7, 2011·Applied Microbiology and Biotechnology·Peter Roslev, Annette S Bukh
Feb 9, 2011·Journal of Water and Health·Melanie WickiAndreas Baumgartner
Feb 18, 2011·Water Research·Daniel Ekane NnaneHuw David Taylor
Nov 4, 2011·Environmental Science & Technology·Sarah E PurnellHuw D Taylor
Jul 28, 2013·Water Research·Valerie J HarwoodMelanie Wicki
Jan 28, 2014·Applied and Environmental Microbiology·Natalie PrystajeckyJudith L Isaac-Renton
Apr 15, 2014·Letters in Applied Microbiology·B R McMinnN J Ashbolt
Citations
Mar 18, 2017·Letters in Applied Microbiology·B R McMinnA Korajkic
Feb 10, 2018·Journal of Water and Health·D DistonR Felleisen
Dec 10, 2020·Frontiers of Environmental Science & Engineering·Mengzhi JiQiang Li