Occurrence of diphthamide in archaebacteria.

Journal of Bacteriology
A M PappenheimerJ W Bodley

Abstract

We examined the nature of the diphtheria toxin fragment A recognition site in the protein synthesis translocating factor present in cell-free preparations from the archaebacteria Thermoplasma acidophilum and Halobacterium halobium. In agreement with earlier work (M. Kessel and F. Klink, Nature (London) 287:250-251, 1980), we found that extracts from these organisms contain a protein factor which is a substrate for the ADP-ribosylation reaction catalyzed by diphtheria toxin fragment A. However, the rate of the reaction was approximately 1,000 times slower than that typically observed with eucaryotic elongation factor 2. We also demonstrated the presence of diphthine (the deamidated form of diphthamide, i.e., 2-[3-carboxyamide-3-(trimethylammonio)propyl]histidine) in acid hydrolysates of H. halobium protein in amounts comparable to those found in hydrolysates of similar preparations from eucaryotic cells (Saccharomyces cerevisiae and HeLa). Diphthine could not be detected in hydrolysates of protein from the eubacterium Escherichia coli. Whereas both archaebacterial and eucaryotic elongation factors contain diphthamide, they differ importantly in other respects.

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Citations

Aug 19, 2004·The Journal of Biological Chemistry·René JørgensenGregers Rom Andersen
May 8, 2008·Cellular Microbiology·Pradeep K GuptaStephen H Leppla
Jan 1, 1985·Critical Reviews in Microbiology·J W Foster, D M Kinney
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Jun 5, 2014·Human Molecular Genetics·Yi-Ru YuChun-Ming Chen
Aug 27, 2013·Critical Reviews in Biochemistry and Molecular Biology·Xiaoyang SuHening Lin
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Dec 23, 2011·Journal of the American Chemical Society·Xiaoyang SuHening Lin
Jan 16, 2014·Journal of the American Chemical Society·Min DongHening Lin

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