Occurrence of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae isolates

PloS One
Peng WangYingyuan Zhang

Abstract

Adequate detection of the production of carbapenemase in Enterobacteriaceae isolates is crucial for infection control measures and the appropriate choice of antimicrobial therapy. In this study, we investigated the frequency of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae clinical isolates by the modified Hodge test (MHT). Three hundred and one E. coli and K. pneumoniae clinical isolates were investigated. All produced extended spectrum β-lactamases (ESBLs) but were susceptible to carbapenems. Antimicrobial susceptibility testing was performed by the disk diffusion and agar dilution methods. The MHT was performed using the standard inoculum of test organisms recommended by the CLSI. Genes that encoded ESBLs and carbapenemases were identified by PCR and DNA sequencing. Among the 301 clinical isolates, none of the isolates conformed to the criteria for carbapenemase screening recommended by the CLSI. The susceptibility rates for imipenem, meropenem, and ertapenem all were 100.0%, 100.0%, and 100.0%, respectively. Of the 301 E. coli and K. pneumoniae isolates, none produced carbapenemase. The MHT gave a positive result for 3.3% (10/301) of the isola...Continue Reading

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Citations

Apr 4, 2014·Canadian Journal of Microbiology·Yu-Zhong YanYi Shi
Sep 10, 2013·PloS One·Qiang ZhangChangjun Zhou
Jun 17, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C VogneG Greub
Aug 8, 2015·Journal of Applied Microbiology·J Osei SekyereS Y Essack
May 2, 2012·Annals of Clinical Microbiology and Antimicrobials·Lijun WangXinxin Lu
Aug 31, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mahmoud A F KhalilMohammed El-Mowafy
May 19, 2017·Journal of Clinical Microbiology·Nicholas M MooreUNKNOWN Carba-R Study Team
Aug 2, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Haiquan KangBing Gu
Feb 13, 2018·Expert Review of Molecular Diagnostics·Molly E FleeceEric R Houpt
Jul 24, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wen-Xia ZhangJue Zhang

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST

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